弓形虫体外感染大鼠心肌细胞的实验研究

目的　观察弓形虫速殖子体外感染大鼠心肌细胞及在细胞内的增殖。方法　用弓形虫RH株速殖子感染体外培养的大鼠心肌细胞,观察虫体在不同的孵育时间对心肌细胞的侵袭率及每个感染细胞内的平均虫数。结果　虫体最早感染心肌细胞的时间为10min ,对心肌细胞的侵袭率随着感染时间的延长而增大。弓形虫侵入心肌细胞后的迟滞期约为8h ,虫体在细胞内增殖一倍的时间约8h。结论　弓形虫感染大鼠心肌细胞及其在细胞内的增殖,为阐述弓形虫对心肌细胞的易感性提供了重要的基础资料。

The experimental study on the rat cardiomyocyte invasion by Toxoplasma gondii in vitro

To investigate the rat cardiomyocyte invasion by Toxoplasma gondii tachyzoites and their replication in the cells.The cultivated rat cardiomyocytes were infected in vitro with RH strain Toxoplasma gondii tachyzoites. Systematic examination on the invasion rates and intracellular multiplication was performed at different incubation time. The results shwed that the earliest time for the tachyzoites invaded the cardiomyocytes was at 10 min. The invasion rates were increased along with the prolonged duration of infection. Tachyzoites displayed an 8 hours lag phase of non-multiplication period. The doubling time of RH strain parasites was approximately 8 hours. In conclusion,the cardiomyocyte invasion by Toxoplasma gondii tachyzoites and parasite multiplication in these cells will provide the basic data for explaining the susceptibility of the cardiomyocytes to the infection with Toxoplasma gondii tachyzoites.