PTEN和VEGF在肺癌中的表达及意义

目的研究PTEN和血管内皮生长因子（VEGF）在肺癌中的表达、临床意义及其相关性.方法应用组织微阵列仪制作肺癌组织芯片.用免疫组织化学S-P法检测PTEN、VEGF在76例肺癌和30例正常肺组织中的表达.结果肺癌组织中PTEN蛋白阳性表达率显著低于正常肺组织（P＜0.01）,VEGF蛋白阳性表达率显著高于正常肺组织（P＜0.01）;PTEN、VEGF的表达与组织分化程度、淋巴结转移和临床病理分期有关;两者表达呈负相关（P＜0.01）.结论联合检测PTEN、VEGF对肺癌的恶性程度及预后判断具有重要的临床意义,应用组织芯片大规模高效检测临床组织样本具有快速、准确、方便经济的特点.

Expression and clinical significance of PTEN and VEGF proteins in human lung carcinoma

Objective: To investigate the expressions and clinical significance of PTEN and vascular endothelial growth factor(VEGF) proteins in human lung carcinoma and the relationship between their expressions.Methods: Tissue microarray technique was used to prepare for tissue microarray with 76 cases of lung carcinoma and 30 cases of normal lung tissue.The expressionsof PTEN and VEGF proteins were detected using immunohistochemical S-P method.Results: The expressions of PTEN and VEGF proteins in cancer tissues were significant difference to theirs in normal tissue(P<0.01).The expressions of PTEN and VEGF proteins were significantly correlated with cell differentiation,lymph node metastasis and clinical stages.The expression of PTENwas negatively related to the expression of VEGF in lung carcinoma(P<0.01).Conclusion: Combined detection of PTEN and VEGF may be helpful to evaluate malignant degree and prognosis of lung carcinoma.Tissue microarray is rapid,economicand accurate to screen the clinical tissue specimens on a large scale.