单采血浆相关性疟疾合并艾滋病病毒感染的研究

目的了解单采血浆相关性疟疾病例中,艾滋病病毒(HIV)感染情况。方法对1993-2005年间单采血浆相关性疟疾病例和非疟疾既往单采血浆供血者进行调查和采集血标本,采用快速蛋白印迹试验或酶联免疫吸附试验检测HIV抗体,初筛阳性者再用蛋白印迹试验进行确认;对现症疟疾病例的血标本用吉氏液染色-光学显微镜(油镜)检查疟原虫。结果 220例单采血浆相关性疟疾病例的HIV感染率为30.0%(66/220),3 008例非疟疾单采血浆供血者的HIV感染率为2.4%(72/3 008)。1993年12月和1994年1月采集并检测的上述两人群,HIV抗体均为阴性;而1995年3月至2005年采集并检测的上述两人群,HIV阳性率则分别为43.4%(66/152)和2.4%(72/2 958)。单采血浆相关性疟疾为间日疟,感染的HIV为HIV-1型。结论单采血浆相关性疟疾病例的HIV感染率较高。

Study on infection of human immunodeficiency virus among cases of plasmapheresis-borne malaria

Objective To understand characteristics of human immunodeficiency virus(HIV) infection among cases of plasmapheresis-borne malaria.Methods Two hundred and twenty cases of plasmapheresis-borne malaria occurred between 1993 and March 1995,and 3 008 former plamapheresis donors were investigated and their blood specimens were collected.Staining of giemsa-optical microscope(oil lens) inspection method was used to detect plasmodium.HIV antibodies were detected by the screening assay of ELISA and confirming assay of Western blot.Results The rates of HIV infection was 30.0%(66/220)for cases of plasmapheresis-borne malaria and 2.4%(72/3 008) for plasmapheresis donors without history of malaria.Sera samples taken from the above two population groups in December 1993 and January 1994 were negative for HIV antibody.Moreover,positive rate of HIV antibody of sera taken in March 1995 to 2005 were 43.42%(66/152)and 2.43%(72/2 958) respectively.The type of plasmapheresis-borne malaria was tertian malaria and that of the infected virus was HIV-1.Conclusion The infection rate of HIV was higher among malaria cases caused by plasmapheresis cross-infection.