Nuclisens EasyQ HIV-1和COBAS AMPLICOR HIV-1 MONITORTM Test检测集合在HIV-1“窗口期”诊断的比较应用 |
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引用本文: | 职建军,潘品良,沈圣,江华洲,郭天骅,段松,王继宝,杨锦,杨艳玲,蒋岩. Nuclisens EasyQ HIV-1和COBAS AMPLICOR HIV-1 MONITORTM Test检测集合在HIV-1“窗口期”诊断的比较应用[J]. 中国艾滋病性病, 2012, 0(4): 210-213 |
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作者姓名: | 职建军 潘品良 沈圣 江华洲 郭天骅 段松 王继宝 杨锦 杨艳玲 蒋岩 |
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作者单位: | 中国疾病预防控制中心性病艾滋病预防控制中心;安阳市疾病预防控制中心;德宏傣族景颇族自治州疾病预防控制中心 |
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基金项目: | 国家“十一五”国家科技重大专项——我国艾滋病流行规律、疫情评估和预测方法研究(2008ZX10001-003);北京市科委项目——HIV-1感染“窗口期”诊断技术和新发感染检测技术研究(D09050704090905)~~ |
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摘 要: | 目的将Nuclisens EasyQ HIV-1方法与COBAS AMPLICOR HIV-1MONITORTM Test方法检测集合的结果进行比较,并将集合HIV RNA Nuclisens EasyQ方法应用于艾滋病病毒Ⅰ型(HIV-1)"窗口期"的检测。方法采用50:1、10:1二级集合HIV RNA COBAS和EasyQ方法进行检测。结果 (1)两种方法在54份定值血清和质控血浆的阳性检出率均为100%,方法的一致性为94.44%,两方法之间具有显著相关性,相关系数为r=0.855。(2)检测感染者配偶、静脉吸毒者(IDU)及男男性行为人群(MSM)样本集合2 426人份,两种方法检测结果一致,发现3例"窗口期"感染者。结论集合HIV RNA Nuclisens EasyQ方法具有很好的实验性能,可应用于HIV高危人群的"窗口期"检测。
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关 键 词: | 艾滋病病毒 窗口期 集合核酸检测 |
Comparative application of Nuclisens EasyQ HIV-1 and COBAS AMPLICOR HIV-1 MONITORTM Test in pooled test for detection of HIV-1 window period |
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Affiliation: | ZHI Jian-jun,PAN Pin-liang,SHEN Sheng,et al.(National Center for AIDS/STD Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China) |
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Abstract: | Objective To compare the results of Nuclisens EasyQ HIV-1 and COBAS AMPLICOR HIV-1 MONITORTM Test in pooled test and to explore the application of HIV RNA Nuclisens EasyQ method in detecting HIV-1 window period.Methods Two levels(50:1,10:1) of pooled method were used.Results(1) 54 valid value sera and quality control plasma were tested,the positive detection rates of the two methods were 100%,the consistency rates of them were 94.44%,and the correlation was significant with a correlation coefficient of R= 0.855.(2) 2426 samples from spouses of HIV infectors,IDUs and MSM were tested;the two methods had consistent results and 3 HIV infected individuals were detected during the window period.Conclusion HIV RNA Nuclisens EasyQ pooled test had good performance and could be applied in detecting window period in HIV high-risk population groups. |
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Keywords: | Human immunodeficiency virus Window period Pooled NAAT |
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