早孕人子宫蜕膜、绒毛膜糖复合物的组化分析

以糖特异性单克隆抗体作为组化探针，观察早孕期人子宫蜕膜、绒毛膜表面糖复合物的变化。结果表明：６种糖特异单抗ＳＨ－１（抗鼠ＬｅＸ单克隆抗体），ＡＨ－６（抗鼠ＬｅＹ单克隆抗体），ＮＵＨ－１（抗鼠６ｃ单克隆抗体），ＮＵＨ－２（抗鼠ＤＳＩ单克隆抗体），Ｊ１（抗鼠Ｔ单克隆抗体），Ｃ６（抗鼠Ｉ单克隆抗体）在早孕期与子宫蜕膜及绒毛膜结合显示不同变化。其中ＡＨ－６、ＳＨ－１绒毛膜滋养细胞结合随妊娠天数增加着色逐渐减弱，相反ＮＵＨ－２的着色逐渐加强。ＡＨ－６，ＳＨ－１，Ｃ６与子宫蜕膜在早孕着色程度无改变。Ｊ１与蜕膜在各期未见结合。ＮＵＨ－１与蜕膜结合随妊娠天数增加而着色逐渐加强。早孕期人子宫蜕膜、绒毛膜糖复合物的变化为进一步研究其功能提供新方法。

Histochemical studies on glycoconjugates of decidua and chorion during early pregnancy

Objective: To study the changes of glycoconjugates in human decidua and chorion during early pregnancy. Method: Immunohistochemical method using six kind of monoclonal antibodies. including AH 6, SH 1,NUH 1,NUH 2,J 1,C 6 Resultes: The binding of AH 6,SH 1 with chorionic trophoblast became weaker along with the increase of gestation days. On the contrary, reactivity of NUH 2 became stronger. AH 6, SH 1,C 6 were strongly expressed on decidua, but did not change at any stage. The J 1 was not showed during whole stage. The binding of NUH 1 with decidua became stronger along with the increase of gestation days. Conclusion: This study suggested the changes of glycoconjugates in human decidua and chorion during early pregnancy, in addition to offer a new method to study its function.