长托宁对缺血/再灌注大鼠肺脏ICAM-1表达的影响

目的探讨长托宁（盐酸戊乙奎醚）对在体肺脏ICAM-1表达的影响。方法SD大鼠36只，随机分为3组：假手术（SO）组、缺彬再灌注（VR）组、长托宁组。左侧开胸，建立肺脏I／R模型。应用长托宁，以免疫组化方法观察肺脏黏附分子（ICAM-1）表达，测定肺髓过氧化物酶（MPO）及超氧化物歧化酶（SOD）活性，以及肺湿质量／干质量比值（W／D）。结果给予长托宁后肺脏组织ICAM-1表达显著降低，MPO活性显著降低，SOD活性增高。结论长托宁可抑制VR后肺脏ICAM-1的表达，从而减少局部白细胞浸润，减轻肺脏结构损害，减轻肺水肿。

Effects of penehyclidine hydrochloride on the expression of ICAM - 1 in rats after lung ischemia - reperfusion

Objective The aim of this study was to investigated the effect of Penehyclidine hydrochloride on the expression of ICAM - 1 in lung in vivo after lung ischemia - reperfusion in rats.Methods Thirty - six male Spragne - Dawley rats were randomized to three groups (twelve control,twelve ischemia-repedusion,twelve after Penehyclidine Hydrochloride).I/R injury consist of 45 min of lung cross -clamping followed by 5 hours of reperfusion;after Penehyclidine Hydrochloride preceding I/R;control animals had a thoracotomy only.Lung tissue wet - to - dry weight ratio and myeloperoxidase concentration were markers of edema and neutrophil se- questration,respectively.Superoxide dismutase activity were assessed,also.Expression of ICAM-1 in lung was measured using im- munohistochemistry,observing the changing of lung tissue in pathology under Electron Microscopy and Light Microscopy.Result Ex- pression of ICAM - 1 significantly decreased after Penehyclidine Hydrochloride,the myeloproxidase activity decreased too,and super- oxide dismutase activity increased.Conclusion Penehyclidine Hydrochloride could inhibit the expression of ICAM-1,and reduce neutrophil -infiltration in lung,and attenuate uhrastructure injury of lung tissue,then attenuate pulmonary edema.