Isolation and characterization of flagella from Trichomonas vaginalis |
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Authors: | Philip F Jemilohun |
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Institution: | (1) Department of Biology, Jackson State University, 1400 J.R. Lynch Street, Jackson, MS 39217, USA Fax: +1-601-974-5853, US |
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Abstract: | As a first step in the biochemical and biomedical analyses of flagella from Trichomonas vaginalis the flagella were isolated, purified, and analyzed. The flagella were detached by mechanical shearing and a crude flagellar
preparation was obtained by low-speed differential centrifugation. The crude flagellar preparation was subjected to further
purification by discontinuous sucrose density-gradient centrifugation. Electron micrographs (EM) of the purified flagella
showed the typical 9 + 2 axonemal arrangement. The structural integrity and the flagellar membrane were not destroyed by the
deflagellation method or the purification scheme employed. The flagellar preparations were resolved by sodium dodecyl sulfate-polyacrylamide
gel electrophoresis. The purified preparation contained many flagellar enriched proteins ranging from 20 to 120 kDa. Three
major proteins of 65 kDa and a doublet of about 50–58 kDa were observed. The protein patterns and EM appearance of the fractions
were highly reproducible.
Received: 12 December 1997 / Accepted: 5 June 1998 |
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Keywords: | |
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