| 荧光原位杂交检测尿脱落细胞染色体异常诊断膀胱肿瘤的研究 |
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| 引用本文： | 王鹏,靳风烁,叶锦,吴刚,李彦锋,张尧,梁平.荧光原位杂交检测尿脱落细胞染色体异常诊断膀胱肿瘤的研究[J].第三军医大学学报,2009,31(10). |
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| 作者姓名： | 王鹏 靳风烁 叶锦 吴刚 李彦锋 张尧 梁平 |
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| 作者单位： | 第三军医大学大坪医院野战外科研究所泌尿外科,重庆,400042 |
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| 摘 要： | 目的 探讨通过荧光原位杂交(fluorescence in situ hybridization,FISH)检测3、7、9、17号染色体数目畸变诊断国人膀胱尿路上皮癌的可行性和有效性.方法 采用3、7、17号染色体着丝粒探针和9p21区带探针对140例患者尿液脱落细胞核行荧光原位杂交,并同时行经腹部超声和尿脱落细胞学检查,以膀胱镜病理检企确诊,比较分析各种方法的敏感性和特异性.结果 ①肿瘤组80例膀胱尿路上皮癌全部经病理检杏确诊,其中68例FISH结果阳性,26例尿脱落细胞学阳性,71例经腹部超声诊断为膀胱癌.②非肿瘤对照组30例,其中29例FISH结果阴性,1例假阳性;尿脱落细胞学检查全部阴性;绛腹超声检查23例阴性,7例假阳性.FISH、尿脱落细胞学、经腹部超声的灵敏度分别为85.00%、32.50%、88.75%,特异度分别为96.67%、100.00%、76.67%.结论 使用FISH技术检测尿脱落细胞3、7、9、17号染色体数目畸变具有敏感度高、特异性强的优点,并具无创性,对国人膀胱尿路上皮癌的诊断具有重要的临床应用价值.
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| 关 键 词： | 膀胱肿瘤 原位杂交 诊断 |
Chromosome aberration of urine exfoliated cells to diagnose bladder neoplasm by fluorescence in situ hybridization |
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| WANG Peng,JIN Feng-shuo,YE Jin,WU Gang,LI Yan-feng,ZHANG Yao,LIANG Ping.Chromosome aberration of urine exfoliated cells to diagnose bladder neoplasm by fluorescence in situ hybridization[J].Acta Academiae Medicinae Militaris Tertiae,2009,31(10). |
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| Authors: | WANG Peng JIN Feng-shuo YE Jin WU Gang LI Yan-feng ZHANG Yao LIANG Ping |
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| Institution: | WANG Peng,JIN Feng-shuo,YE Jin,WU Gang,LI Yan-feng,ZHANG Yao,LIANG Ping(Department of Urology,Institute of Surgery Research,Daping Hospital,Third Military Medical University,Chongqing 400042,China) |
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| Abstract: | Objective To evaluate the possibility and validity of detecting numerical aberration of chromosomes 3,7,9 and 17 by fluorescence in situ hybridization(FISH)in order to diagnose urothelial carcinoma of bladder in Chinese.Methods A total of 140 volunteers were recruited as subjects,including 80 hematuria patients suffering from bladder urothelial carcinoma,30 hematuria patients with benign bladder diseases,and 30 healthy individuals.Exfoliated cells from fresh urinary specimen was collected and examined by FI... |
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| Keywords: | bladder neoplasm in situ hybridization diagnosis |
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