江西乙型肝炎病毒基因型分布及其与临床的相关性 |
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引用本文: | 张华,温志立,叶倩,张伦理,邬小萍,向天新. 江西乙型肝炎病毒基因型分布及其与临床的相关性[J]. 实用肝脏病杂志, 2009, 12(3): 184-186,200 |
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作者姓名: | 张华 温志立 叶倩 张伦理 邬小萍 向天新 |
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作者单位: | 1. 第一医院感染性疾病科,南昌市,330008 2. 330008,南昌市,第一医院感染性疾病科;中南大学湘雅医学院博士后流动站 3. 南昌市卫生学校 4. 南昌大学第一附属医院感染病科 |
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基金项目: | 江西省南昌市科技局重点攻关项目 |
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摘 要: | 目的了解江西慢性乙型肝炎患者血清乙型肝炎病毒(HBV)基因型的分布情况及HBV基因型与临床的关系。方法选择HBV DNA阳性的慢性乙型肝炎患者血清150份,患者分别诊断为慢性HBV携带者、慢性肝炎轻度、中度、重度和慢性重型肝炎,每组30例,采用多对型特异性引物巢式PCR法检测HBV基因型。结果本组慢性乙型肝炎患者HBV基因分型结果为B型118例(78.7%),C型31例(20.7%),D型1例(0.6%);在上述5组患者中C型比例分别为0%,16.7%,26.7%,30.0%和30.0%,其中慢性HBV携带者与其他组比,差异有统计学意义(P〈0.05);B、C两基因型患者HBeAg阳性率分别为83.9%(99/118)和90.3%(28/31),HBeAb阳性率分别为16.1%(19/118)和9.7%(3/31),差异均无统计学意义(P〉0.05)。结论江西地区HBV优势基因型以B型为主,C型次之,有少量的D型感染者。
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关 键 词: | 乙型肝炎 乙型肝炎病毒基因型 多对型特异性引物 巢式PCR |
Detection of hepatitis B viral genotypes in Jiangxi Province of China by nested PCR with multiplex pairs of primers |
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Affiliation: | ZHANG Hua,WEN Zhili,YE Qian,et al. (Department of Infectious Diseases,the First Hospital,Nanchang) |
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Abstract: | Objective To determine the hepatitis B virus (HBV) genotypes in Jiangxi Province by nested PCR with multiplex pairs of genotype-specific primers. Methods 150 serum samples from HBV DNA positive patients with chronic heptitis B virus infection were divided into 5 groups according to patient's diagnosis,with each group of 30 samples. The 5 groups were asymptomatic carrier,mild,moderate,severe hepatitis B and fulminant hepatitis B,respectively. Nested PCR with multiplex pairs of genotype-specific primers was used to determinate the genotypes of HBV. Results Of the 150 patients,118 (78.7%) were with genotype B,31 (20.7%) with genotype C and 1 (0.6%) with genotype D. The positive rates of HBV genotype C in patients with asymptomatic carrier,mild,moderate,severe hepatitis B and fulminant hepatitis B were 0%,16.7%,26.7%,30.0% and 30.0%,respectively,which was significantly different (P〈0.05) only between asymptomatic group and others. The HbeAg positivity in patients with genotype B and C infection were 83.9% (99/118)and 90.3%(28/31),respectively and the HBeAb positivity were 16.1%(19/118) and 9.7%(3/31),respectively (P〉 0.05). Conclusion The predominant HBV genotypes in Jiangxi Province are confirmed to be genotypes B and C. |
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Keywords: | Hepatitis B Hepatitis B viral genotype Nested PCR Multiplex pairs of genotype-specifie primers |
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