黄芩素诱导人子宫颈癌HeLa细胞凋亡

目的:探讨黄芩素对人子宫颈癌HeLa细胞凋亡的影响及其可能的机制。方法:MTT法测定黄芩素作用后人子宫颈癌HeLa细胞的生长抑制率;Hoechst 33258荧光染色法和Annexin-V/PI双染流式细胞术检测黄芩素诱导HeLa细胞凋亡的作用;Westernblot观察黄芩素对HeLa细胞bcl-2、Bax、caspase 3及p53表达的影响。结果:(1)不同浓度黄芩素作用24h后均可抑制HeLa细胞生长(P<0.05);(2)黄芩素作用24h后,HeLa细胞呈典型的凋亡形态学改变,凋亡率亦显著增加(P<0.01);(3)细胞内Bax、p53和caspase 3表达显著上调,bcl-2表达明显下调(P<0.05)。结论:黄芩素通过调控凋亡相关基因的表达,诱导HeLa细胞凋亡。

Induction of apoptosis by baicalein on human cervix cancer HeLa cells

Objective:To investigate the effect of baicalein on cell apotosis of human cervix cancer HeLa cells and the possible mechanism.Methods:MTT assay was used to determine the cell growth inhibitory rate.Cellular apoptosis was detected by Hoechst 33258 fluorescent staining and Annexin-V/PI double-staining assay via flow cytometric analysis.The expressions of bcl-2,Bax,p53 and caspase 3 were determined by Western blot.Results:(1)After the treatment with different concentration of baicalein for 24 hours,HeLa cell growth was significantly inhibited(P<0.05).(2)The apoptosis rates of HeLa cells were significantly increased in response to baicalein treatment(P<0.01).(3)The expressions of Bax,p53 and caspase 3 were up-regulated(P<0.05 to P<0.01),but the expression of bcl-2 was down-regulated when HeLa cells were treated with baicalein for 24 hours.Conclusion:Baicalein can induce apoptosis of HeLa cells probably by regulating the expressions of gene assioated with apoptosis.