巨噬细胞对卵巢癌细胞SKOV3生物学功能的影响

目的:研究巨噬细胞对卵巢癌细胞株SKOV3生物学功能的影响。方法:(1)体外采用IL-4和佛波醇酯(PMA)分别诱导M2和M1型巨噬细胞,流式细胞仪鉴定分型;(2)Tranwell小室建立巨噬细胞与卵巢癌细胞SKOV3体外非接触式共培养模型。比较共培养后,SKOV3的增殖和凋亡、迁移和侵袭的变化。MTT法检测增殖;流式细胞仪Annexin V-FITC/PI双染检测凋亡;Transwell检测侵袭和迁移。结果:(1)IL-4诱导的巨噬细胞高表达CD163,为M2型,PMA诱导组高表达HLA-DR,为M1型。SKOV3和普通巨噬细胞共培养后,巨噬细胞CD163高表达。(2)SKOV3的增殖和凋亡:M2共培养组SK-OV3的增殖活性显著高于M1共培养组和普通共培养组(P<0.05)。M2共培养组SKOV3的凋亡率显著低于M1共培养组和普通共培养组(P<0.05)。(3)SKOV3的迁移和侵袭:M2共培养组SKOV3的侵袭能力显著强于M1共培养组和普通共培养组(P<0.01)。M2共培养组SKOV3的迁移能力显著强于M1共培养组和普通共培养组(P<0.05)。结论:共培养卵巢癌细胞使巨噬细胞M2表型极化。M2型巨噬细胞促进卵巢癌细胞增殖,提高侵袭、迁移能力,减少凋亡,而M1型起相反作用。

Influence of tumor associated macrophages on biological function of SKOV3 cell

Objective:To investigate the influence of tumor-associated macrophages(TAMs) on the biological function of human ovarian cell line SKOV3.Methods:Macrophage was induced into M2 subtype macrophage form with interleukin(IL)-4 plus PMA,M1 subtype with LPS plus PMA respectively,macrophage scarenger receptor(CD163) were analyzed with flow cytometry.SKOV3 was co-cultured with TAMs in the transwell.Apoptosis and proliferation of SKOV3 were detected with MTT and Annexin V-PI flow cytometry,.migration and invasion capability were measured by transwell assay respectively.Results:Coculturing with SKOV3 induced M2 subtype macrophage with elevated expression of CD163.After co-cultured with M2 macrophage,the activity of apoptotic rates decreased and the proliferation,the migration and invasion capability increased for cocultured SKOV3.Conclusion:Cocultured with SKOV3,macrophages tended to polarized into a M2 subtype,M2 macrophage may contribute to cancer progression by inhibiting the apoptosis while promoting the proliferation,migration and invasion capability of SKOV3.while M1 macrophage plays an opposing effect.