血府逐瘀汤促进大鼠随意皮瓣存活的疗效

目的：研究血府逐瘀汤对大鼠随意皮瓣存活的疗效，并初步探究合适的剂量。方法：取72只雄性SD大鼠，建立改良大鼠McFarlane flap模型，将实验动物随机分为4组，每组18只：对照组：0.9%氯化钠溶液10 mL·kg-1·d-1灌胃；血府逐瘀汤高剂量组（高剂量组）：生药13 g·kg-1·d-1灌胃；血府逐瘀汤中剂量组（中剂量组）：生药6.5 g·kg-1·d-1灌胃；血府逐瘀汤低剂量组（低剂量组）：生药3.25 g·kg-1·d-1灌胃。连续用药7 d后，分别测算各组的皮瓣存活面积，并处死动物取皮瓣组织做HE染色，评估组织内微血管密度（MVD），Western blot检测组织内血管内皮生长因子（VEGF）表达量，用相应试剂盒检测超氧化物歧化酶（SOD）及丙二醛（MDA）含量。结果：皮瓣术后7 d，不同剂量血府逐瘀汤治疗组的皮瓣存活面积相较对照组均明显增加（P ＜0.01），HE染色显示MVD明显增加（P ＜0.01），VEGF表达量明显升高（P ＜0.05）。其中，中剂量组的治疗效果最为明显（P ＜0.05）。另外，不同剂量组的皮瓣内SOD表达量较对照组明显增加（P ＜0.05），MDA表达量明显减少（P ＜0.05）。结论：血府逐瘀汤可促进大鼠随意皮瓣的存活，其中，中剂量组治疗效果最佳。其机制可能是通过促进缺血组织内的VEGF表达，从而增加组织内的新生血管密度，促进微循环重建，同时抑制组织的缺血再灌注损伤。

Experimental study on the effect of Xuefu Zhuyu Tang on the survival of random skin flaps

Objective: To invetigate the effect of Xuefu Zhuyu Tang (XZT) on the survival of random skin flaps and to make clear of its appropriate dose. Methods: Totally 72 male SD rats were used to establish a modified McFarlane flap model. Then the animals were randomly divided into 4 groups (n=18): control group with 10 mL·kg-1·d-1 saline by gavage; high-dose XZT group (high-dose group) with 13 g·kg-1·d-1 crude drug by gavage;medium-dose XZT group (medium-dose group) with 6.5 g·kg-1·d-1 crude drug by gavage andlow-dose XZT group (low-dose group) with 3.25 g·kg-1·d-1 crude drug by gavage. Seven days after continuous administration,the survival area of random skin flaps in each group was measured, and the tissue samples were harvested for histological (HE) staining, by which the micro-vessel density (MVD) was evaluated. Western blotting (WB) was used to detect the expression of (vascular endothelial growth factor) VEGF. And the (superoxide dismutase) SOD and (malondialdehyde) MDA content in tissues were evaluated. Results: At 7 days after operation, the survival areas of flaps in the different doses XZT groups were significantly higher than in the control group (P<0.01).HE staining showed an obvious increase in micro-vessel density (P<0.01), and VEGF expression was improved(P<0.05). Among them, the middle-dose group has the most obvious therapeutic effect. In addition, the content of SOD in the flaps of different dose XZT groups was significantly higher than that in the control group, and MDA was reduced (P<0.05). Conclusion: XZT can promote the survival of rat random flaps, with the most obvioustherapeutic effect shown by the middle-dose group. The underlying mechanism of XZT for the survival of random skin flaps probably lies in that the drug promotes VEGF expression in ischemic tissue, thereby increasing neovascularization in the tissue and promoting microcirculation reconstruction while inhibiting ischemiareperfusioninjury.