运动训练调节PI3K/AKT信号通路抑制凋亡改善大鼠脑缺血再灌注损伤研究

目的 探讨运动训练通过调节PI3K/AKT信号通路抑制凋亡改善大鼠脑缺血再灌注损伤的作用。方法 36只雄性SD大鼠随机分为3组, 即假手术组(Sham)、模型组(MCAO)及运动训练组(EX+MCAO), 每组12只。运动训练采用跑台跑步方法, 在术后24小时进行跑台训练, 连续4周。采用线栓法制备大鼠大脑中动脉栓塞(middle cerebral artery occlusion, MCAO)模型, 运动训练后分别检测各组大鼠神经功能评分, TTC染色观察脑梗死体积, 用HE染色检测脑组织损伤, Tunnel染色检测细胞凋亡, Western blot法检测海马组织PI3K、p-PI3K、AKT和p-AKT蛋白表达水平。结果 与Sham组比较, MCAO组大鼠神经功能评分显著增加, 脑梗死体积明显增大, 脑损伤加重, p-PI3K和p-AKT蛋白表达水平显著降低。与MCAO组比较, 运动训练组大鼠的神经功能损伤明显改善, 脑梗死体积减小, 脑损伤减轻, p-PI3K和p-AKT蛋白表达水平显著升高。结论 运动训练可能通过调控PI3K/AKT信号通路抑制细胞凋亡的过度发生, 从而减轻脑缺血再灌注损伤, 起到脑保护作用。

Study on the effects of exercise training on PI3K/AKT signaling pathway and apoptosis on cerebral ischemia-reperfusion injury in rats

Objective To explore the effect of exercise training on the improvement of cerebral ischemia-reperfusion injury by regulating PI3K/Akt signal pathway and inhibiting apoptosis.Methods 36 male SD rats were randomly divided into three groups:sham operation group (sham), model group (MCAO) and exercise training group (Ex + MCAO).The treadmill running method was used in the sports training, and the treadmill training was carried out 24 hours after the operation for 4 consecutive weeks.The middle cerebral artery occlusion (MCAO) model was made by the method of thread embolism.After exercise training, the neurological function scores of each group were measured, the volume of cerebral infarction was observed by TTC staining, the brain injury was detected by HE staining, the apoptosis was detected by tunnel staining, and the expression of PI3K, p-PI3K, Akt and p-Akt protein in hippocampus was detected by Western blot.Results Compared with sham group, MCAO group significantly increased neurological function score, cerebral infarction volume, brain injury, p-PI3K and p-Akt protein expression level.Compared with the MCAO group, the motor training group significantly improved the neurological damage, decreased the volume of cerebral infarction, reduced the brain damage, and significantly increased the expression of p-PI3k and p-Akt protein.Conclusion Exercise training may inhibit the over occurrence of apoptosis by regulating PI3K / Akt signal pathway, so as to reduce the cerebral ischemia-reperfusion injury and play a role in brain protection.