紫苏梗影响结肠平滑肌细胞收缩作用的实验研究

目的]观察紫苏梗水提液对肢体缺血再灌注大鼠离体结肠平滑肌细胞运动的影响,并探讨其作用机制.方法]建立肢体缺血/再灌注大鼠胃肠动力障碍模型.酶法分离大鼠结肠平滑肌细胞,采用图像分析系统测定细胞长度,利用工具药探讨机制.结果]肢体缺血,再灌注后,结肠平滑肌细胞平均长度明显增加.紫苏梗水提液可明显增加模型大鼠结肠平滑肌细胞的收缩率,最适剂量为50g/L.钙鳌合剂(EGTA)和钙通道阻断剂盐酸维拉帕米均可完全抑制紫苏梗水提液对结肠平滑肌细胞的收缩作用.结论]紫苏梗水提液对肢体缺血再灌注大鼠离体单个结肠平滑肌细胞有明显的收缩作用,其作用主要通过胞外Ca2+内流介导.

Effects of Perilla frutescens stem on contraction of colon smooth muscle cells in rats

Objective] To study the effects of Perilla frutescens stem on the contraction of colon smooth muscle cells of rats with lower limb ischemic reperfusion(LIR) and explore the mechanism. Methods] LIR was induced in Wistar rats. Smooth muscle cells were isolated from the colon of the rat. The contractile response of colonic smooth muscle cells to Perilla frutescens stem and other medicines were observed respectively. Results] The mean length of the colonic smooth muscle cells increased in model rats. Perilla frutescens stem could increase the contraction rate of model colon smooth muscle cells,the concentration of the maximal response was 50 g/L. The contraction caused by Perilla frutescens stem could be inhibited completely by EGTA or verapamil. Conclusion] The contraction caused by Perilla frutescens stem may be mediated by the presence of extracellular calcium.