| 水提-醇沉法提取大黄多糖工艺优化研究 |
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| 引用本文： | 孙莹,纪跃芝,马爱民,纪耀华.水提-醇沉法提取大黄多糖工艺优化研究[J].中国实用医药,2010,5(18):6-8. |
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| 作者姓名： | 孙莹 纪跃芝 马爱民 纪耀华 |
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| 作者单位： | 1. 长春医学高等专科学校,130031
2. 长春工业大学 |
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| 基金项目： | 吉林省教育厅"十一五"科学技术研究项目 |
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| 摘 要： | 目的确定大黄多糖的最佳提取条件。方法以大黄多糖的含量为指标，以提取温度、提取时间、提取次数、料液比为因素，利用正交试验法进行试验设计，对传统水提一醇沉法提取大黄多糖的工艺进行优化研究。结果大黄粗多糖得率最高的提取条件是：提取时间3h，提取温度95℃，料液比1：30；大黄粗多糖纯度最高的条件是：提取时间1h，提取温度95℃，料液比1：10；乙醇沉淀大黄多糖的最佳浓度是80％。结论本方法实验结果可作为提取大黄多糖的工艺制定的依据。
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| 关 键 词： | 大黄 多糖 水提-醇沉法 工艺研究 |
Studies on the process of rhubarb thick polysaccharide tradition through the traditional water solution and alcohol sedimentation |
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| SUN Ying,JI Yue-zhi,MA AI-ming,et al..Studies on the process of rhubarb thick polysaccharide tradition through the traditional water solution and alcohol sedimentation[J].China Practical Medical,2010,5(18):6-8. |
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| Authors: | SUN Ying JI Yue-zhi MA AI-ming |
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| Institution: | SUN Ying,JI Yue-zhi,MA AI-ming,et al.Changchun Medical College,Changchun 130031,China |
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| Abstract: | Objective To determine the optimum extracting conditions of rhubarb polysaccharide.Methods By orthogonal test,take the content of rhubarb polysaccharide as standards,extraction temperature,extraction time,extraction times and ratio of material to liquid as factors,to optimize and study the process of extracting rhubarb polysaccharide through the traditional water solution and alcohol sedimentation.Results The optimum extraction condition of rhubarb thick polysaccharide has been established as follows:extrac... |
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| Keywords: | Rhubarb Polysaccharidek Water solution and alcohol sedimentation The process study |
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