甲基化硒酸对人前列腺癌DU145细胞增殖与凋亡的影响

目的：观察甲基化硒酸（MSA）对DU145前列腺癌细胞增殖及凋亡的影响，并初步探讨其作用机制。方法:采用MTT比色法观察1.25、2.50和5.00 μmol?L^-1MSA对DU145细胞增殖活力的抑制作用；吖啶橙染色观察MSA诱导细胞凋亡情况；流式细胞术分析细胞周期及凋亡，并计算细胞平均凋亡指数；免疫细胞化学技术观察细胞内激活的caspase-3的表达情况。结果：经1.25、2.50和5.00　 μmol?L^-1MSA处理48　h后，DU145细胞生长抑制率分别为16.35%、38.10%和73.54%，3组间比较差异有显著性（P<0.01），随MSA浓度升高细胞生长抑制率增加，呈明显的剂量依赖性。吖啶橙荧光染色可见，经1.25、2.50和5.00　 μmol?L^-1MSA处理后的细胞呈明显凋亡形态，并随药物剂量增加凋亡细胞数增多；流式细胞术结果显示，经1.25、2.50和5.00　 μmol?L^-1MSA处理48 h后，细胞凋亡率分别为5.34%、8.71%和13.60%，3组间比较差异有显著性（P<0.05）,随MSA浓度升高细胞凋亡率升高，呈剂量依赖关系；免疫细胞化学染色结果显示，随着MSA剂量增加，细胞内激活的caspase-3表达上调。结论:MSA对DU145细胞具有明显的抗肿瘤作用，其机制可能与激活的caspase途径诱导细胞凋亡有关。

Effect of methylseleninic acid on apoptosis and proliferation of prostatic cancer cells DU145

Objective To observe the effect of methylseleninic acid (MSA) on proliferation and apoptosis of the prostatic cancer cells DU145 and approach the mechanism.Methods After DU145 cells were treated with 1.25,2.50 and 5.00 μmol·L-1MSA,the cell viability was examined by MTT test. The techniques of acridine orange staining and flow cytometry were used to analyze apoptotic cycle and calculate the apoptotic index. The expression of active caspase-3 was detected with immunocytochemistry. Results After DU145 cells were treated with 1.25,2.50 and 5.00 μmol·L-1MSA,the growth inhibitory rates of cell line were 16.35%,38.10% and 73.54 %,respectively,the inhibitory rate increased with the increasing of MSA concentration in a dose-dependent manner. There existed obvious differences between three groups(P<0.01).Acridine orange staining results showed that the cells were apoptotic state when treated with 1.25,2.50 and 5.00 μmol·L-1MSA,the number of apoptotic cells increased with MSA dose increasing. Flow cytometry results showed that when the cells were treated with 1.25,2.50 and 5.00 μmol·L-1MSA for 48 h,the apoptotic rates were 5.34%,8.71% and 13.6%,respectively; there existed obvious differences between three groups (P<0.05).The expression of active caspase-3 was upregulated with MSA dose increasing. Conclusion MSA can induce the apoptosis of prostatic cancer cells DU145 and has significant anti-tumor effect,its mechanism may be related to activate the pathway of caspase.