白萝卜提取物对大鼠胃排空、肠推进及豚鼠离体回肠平滑肌的影响

目的 研究白萝卜提取物（ Raphanus sativus extract）对在体大鼠胃排空及肠推进的影响及对豚鼠离体回肠平滑肌条收缩活动的影响。方法 通过旋转蒸发的方法，制备得到白萝卜提取物；将32只SD大鼠随机分为白萝卜提取物高剂量组（3ml／kg）、中剂量组（1ml／kg）、低剂量（0．3ml／kg）组及空白对照组（生理盐水）4个组，每组8只，通过灌胃给药的方法，以胃肠内标记物葡聚糖蓝-2000在大鼠胃内色素相对残留率及肠推进比为指标，观察各组对大鼠胃排空及肠推进的影响，进一步在离体条件下，以豚鼠回肠平滑肌肌条的收缩幅度和频率为指标，通过不同浓度的白萝卜提取物作用于豚鼠离体回肠平滑肌，观察提取物对离体回肠平滑肌收缩活动的影响。结果白萝卜提取物在0．3ml／kg，1．0ml／kg，3ml／kg剂量下都能够提高大鼠胃排空率，与空白对照组相比，差异具有显著性（P〈0．01）；通过小肠推进比的比较，发现0、3ml／kg、1ml／kg剂量的提取物可明显促进小肠推进，与空白对照组相比，差异具有显著性（P〈0．01）；而3ml／kg剂量下的提取物未表现出明显的促进小肠推进作用，与空白对照组相比，差异无显著性（P〉0．05）。在离体条件下，提取物在0．1mg／ml下对豚鼠离体回肠平滑肌的张力和收缩频率均无明显影响，但可增加其收缩幅度（与给药前相比，P〈0．05）；而在0．3mg／ml，1．0mg／ml，3．0mg／ml剂量下，均可使豚鼠离体回肠平滑肌的张力、收缩幅度增加（与给药前相比，P〈0、01），但不改变收缩频率（与给药前相比，P〉0．05）。结论 白萝卜提取物对动物胃肠动力具促进作用，并存在一定的剂量一效应关系。

Effects of Raphanus sativus extract on gastric emptying and small bowel peristalsis in rats in vivo and smooth muscle of ileum of Guinea pig in vitro

Objective To observe the effects of the Raphanus sativus extract(Ecr) on gastric emptying and small bowel peristalsis activity in rats in vivo and contraction activity of the smooth muscle of Guinea pig in vitro.Methods The Raphanus sativus extract(Ecr)was obtained using rotary evaporation method.Thirty-two SD rats were randomly divided into four groups.The rats in experiment groups were administered with 0.3 ml/kg,1 ml/kg and 3 ml/kg of Ecr with gavage respectively.The rats in normal control group were given distillated water.By observing the residue rates of pigment in stomach and propulsive proportion of small intestine of dextran blue-2000 in rats,the capacity of gastric emptying and small bowel peristalsis induced by Ecr could be studied.Moreover,the samples of isolated ileum segment from guinea pigs were prepared,and the contraction intensity and frequency of the smooth muscle were measured before and after treatment of Ecr.Results Compared with the control group,the Ecr could increase gastric emptying in experiment groups,and there was significant difference(P<0.01).Compared with the control group,the Ecr could also enhance the small bowel peristalsis in 0.3 ml/kg and 1 ml/kg groups(P<0.01),but not in 3 ml/kg group.After the treatment of Ecr,the Ecr could enhance the contraction range in 0.1 ml/kg group,P<0.05,but not change the strain and frequency of the guinea-pig ileum in vitro;While in 0.3,1.0 and 3.0 mg/ml group,the Ecr could enhance the contraction range and strain,butnot change contraction frequency of the guinea-pig ileum in vitro,P<0.01.Ecr showed dose-dependent spasmogenicity on Guinea-pig ileum in vitro.Conclusion The Ecr can promote the gastrointestinal motility,and shows a dose-dependent relationship.