IL-10基因启动子-592A/C多态性与HBV感染预后的关系

摘 要 目的：探讨湖北汉族人白细胞介素-10（IL-10）基因启动子区-592位点单核苷酸多态性与乙型肝炎病毒（HBV）感染、转归的关系。方法：采用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）方法，检测231例慢性HBV感染者（其中慢性乙型肝炎75例，无症状携带156例），165例HBV感染自愈者和135例正常对照者IL-10基因启动子区-592A/C位点基因型。结果：IL-10基因启动子区-592A/C位点基因型和等位基因在慢性HBV感染组、HBV感染自愈组和正常对照组之间的分布频率比较差异无显著性（P>0.05），在血清HBV-DNA<1×103copies/mL的慢性HBV感染者组和HBV-DNA≥1×103 copies/mL组之间的分布频率比较差异亦无显著性（P>0.05）；但IL-10基因启动子区-592位点AA基因型和A等位基因在慢性乙型肝炎组出现的频率明显高于HBV无症状携带组，两组之间分布比较差异有显著性（P<0.05）。结论：汉族人IL-10基因启动子区-592位点多态性可能与人群对HBV易感性及感染后的病毒血症水平无显著相关性，但该位点基因多态性与HBV感染后的肝脏炎症反应有关。≥≥≥≥≥

Relationship between genetic polymorphisms of interleukin-10 gene promoter -592A/C site and hepatitis B virus infection

【Abstract】 Objective To investigate the correlation between polymorphisms of interleukin-10（IL-10）gene promoter region –592 site and the susceptibility and clinical phenotypes of hepatitis B virus（HBV） in Hubei Han population. Methods Using polymerase chain reaction with restriction fragment length polymorphism（PCR-RFLP）method, the single nucleotide polymorphisms（SNP）of the promoter region of IL-10 gene at position -592A/C were detected in 231 patients with chronic hepatitis B(75 patients with chronic hepatitis B and 156 of asymptomatic HBV carriers), 165 individuals spontaneously recovered from HBV infection and 135 normal controls. Results No significant difference was found in frequencies of genotypes and alleles of IL-10 gene promoter region at position -592A/C among normal controls, individuals spontaneously recovered from HBV infection and patients with chronic hepatitis B（P>0.05）, also between patients with HBV infection with HBV-DNA<1×103 copies/mL and those with HBV-DNA≥1×103 copies/mL（P>0.05）. However, frequencies of AA genotype and allele A at position –592A/C in chronic hepatitis B were significantly higher than that in asymptomatic HBV carriers（P<0.05）. Conclusions It is probable that genetic polymorphisms of interleukin-10 promoter region –592 site are not associated with both susceptibility of HBV infection and HBV-DNA replication after infected HBV in Hubei Han population, but are related to inflammatory reaction in the liver of the patients with HBV infection.