产地对蟾酥药材质量的影响

目的: 建立蟾酥药材质量分析方法,并分析产地对其有效成分的影响。 方法: 以华蟾酥毒基和脂蟾毒配基为对照,采用薄层色谱法和高效液相色谱法进行分析和含量测定。 结果: 不同产地蟾酥药材所含成分类型基本相似,但在成分含量上存在明显的差异,以脂蟾毒配基含量变化最为明显,其中产地山东的蟾酥的华蟾酥毒基和脂蟾毒配基含量基本最高;成分差异在薄层色谱图谱中主要表现在斑点2(脂蟾毒配基)、斑点3(华蟾酥毒基)、斑点4、斑点6和斑点9上,在高效液相图谱中表现在3,4,6,7,8,9,14,15号峰上,且还可以看出7,8号峰的含量与14号(华蟾酥毒基)、15号(脂蟾毒配基)峰含量可能存在相关性。 结论: 不同产地蟾酥药材存在明显质量差异;本文方法稳定可行,重现性和专属性好,可用于蟾酥鉴别及质量分析。

Influence of dfferent producing area on quality of Venenum bufonis

Objective: To establish a quality analysis method for Venenum Bufonis and to study influence of different producing area on active composition. Method: TLC and HPLC were applied with analysis and determination of the content of cinobufagin and resibufogenin. Result: Venenum Bufonis from different producing area had the similar compositions. But the content of compositions was significantly different especially for resibufogenin in that the Venenum Bufonis produced in Shandong had the highest content of cinobufagin and resibufogenin. The difference of compositions displayed majorly in spot 2(resibufogenin), spot 3(cinobufagin), spot 4, spot 6 and spot 9 in TLC chromatogram and peak 3, peak 4, peak 6, peak 7, peak 8, peak 9, peak 14 and peak 15 in HPLC chromatogram. The HPLC chromatogram could also see that the content of peak 7, peak 8 and the content of peak 14(cinobufagin), peak 15(resibufogenin)had possible relationship. Conclusion: The quality of Venenum Bufonis from different producing area has significant difference. The established method is stable, feasible with a good reproducibility and specificity, and suitable for identification and quantitative analysis of Venenum Bufonis.