| 高效液相色谱法测定色甘酸钠滴眼液的含量 |
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| 引用本文： | 于盛茂,万秀玉,贺艳丽,刘燕. 高效液相色谱法测定色甘酸钠滴眼液的含量[J]. 中国生化药物杂志, 2002, 23(3): 148-149 |
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| 作者姓名： | 于盛茂 万秀玉 贺艳丽 刘燕 |
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| 作者单位： | 山东省生物药物研究院,山东,济南,250014 |
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| 摘 要： | 目的建立测定色甘酸钠滴眼液含量的高效液相色谱法。方法采用WatersSymmetryC1 8色谱柱 ,甲醇 乙腈 0 .0 5mol/L枸橼酸钠缓冲液 (pH 3 .5) 水 (1 2∶1 0∶40∶38)为流动相 ,检测波长为 32 5nm。结果在 0 .4～ 1 6 .4μg范围内线性关系良好 ,r =0 .9999。此法与紫外分光光度法测得平均回收率分别为 99.88% (RSD =0 .49% )、99.2 6 % (RSD =0 .82 % )。结论此法简单、准确 ,专属性强 ,可用于色甘酸钠滴眼液的含量测定。
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| 关 键 词： | 色甘酸钠滴眼液 高效液相色谱法 含量测定 |
| 文章编号： | 1005-1678(2002)03-0148-02 |
| 修稿时间： | 2001-07-01 |
Determination of sodium cromoglicate eye drops by HPLC |
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| YU Sheng mao,WAN Xiu yu,HE Yan li,LIU Yan. Determination of sodium cromoglicate eye drops by HPLC[J]. Chinese Journal of Biochemical Pharmaceutics, 2002, 23(3): 148-149 |
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| Authors: | YU Sheng mao WAN Xiu yu HE Yan li LIU Yan |
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| Abstract: | PurposeTo establish HPLC method for the determination of sodium cromoglicate eye drops.MethodsChromatography column: Waters Symmetry C 18 , mobile phase: methanol acetonitrile 0.05 mol/L sodium citrate buffer(pH 3.5) water (12∶10∶40∶38),detection wavelength:325nm.ResultsIt was linear with a correlation coefficient of 0.999 9 at the range of 0.4～16.4 μg. The average recoveries were 99.88%( RSD =0.49%), 99.26%( RSD =0.82%) respectively for the HPLC method and UV method.ConclusionThe method was simple,accurate,specific and suitable for the determination of sodium cromoglicate eye drops. |
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| Keywords: | sodium cromoglicate eye drops HPLC determination |
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