| 荧光原位杂交技术在R-显带后玻片上的应用 |
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| 引用本文： | 薄丽津,李承文,秦爽,刘世和. 荧光原位杂交技术在R-显带后玻片上的应用[J]. 现代检验医学杂志, 2006, 21(2): 8-9 |
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| 作者姓名： | 薄丽津 李承文 秦爽 刘世和 |
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| 作者单位： | 中国医学科学院中国协和医科大学血液学研究所血液病医院,天津,300020 |
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| 摘 要： | 目的探讨荧光原位杂交技术(F ISH)在R-显带后玻片上的应用。方法对17例初诊为M 3患者,应用PM L/RAR a探针在R显带标本玻片上直接进行荧光原位杂交检测。结果6例显带质量差的标本中5例确认为PM L/RAR a阳性,1例阴性;2例疑似复杂易位的标本均确证为累及第三条染色体的t(15;17)复杂易位,并精确定位;9例核型检出t(15;17)的标本均证实分子水平上存在PM L/RAR a融合基因。结论R显带联合F ISH方法在复杂变异易位的鉴定方面与传统的核型分析和直接F ISH检测相比具有明显的优势。对于未知异常染色体的检出将具有更广泛的应用前景。
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| 关 键 词： | 染色体 显带 荧光原位杂交 |
| 文章编号： | 1671-7414（2006）02-008-02 |
| 收稿时间： | 2005-10-18 |
| 修稿时间： | 2005-10-18 |
The Utilities of Fluorescence in Situ Hybridization Technique on R-banding Treated Slides |
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| BO Li-jin,LI Cheng-wen,QIN Shuang,LIU Shi-he. The Utilities of Fluorescence in Situ Hybridization Technique on R-banding Treated Slides[J]. Journal of Modern Laboratory Medicine, 2006, 21(2): 8-9 |
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| Authors: | BO Li-jin LI Cheng-wen QIN Shuang LIU Shi-he |
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| Affiliation: | Haematological Disease Hospital, Xiehe Medical University, Chinese Academy of Sciences, Tianjing 300020, China |
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| Abstract: | Objective To investigate the utilities of fluorescence in situ hybridization technique(FISH) on R-banding treated slides.Methods FISH was performed with PML/RARa probes on R-banding treated slides of 17 untreated M3 patients.Results Five positive specimens and one negative specimens were revealed in six specimens of poor quality R-banding slides.Complex aberrant translocations were revealed in 2 specimens and were mapped.The PML/RARa fusion gene was revealed in 9 specimens with t(15;17) karyotype.Conclusion The R-banding with FISH techniques as compared with conventional karyotype analysis and direct FISH test in analysis of complex aberrant translocation.They have widly applied future for unrecognized chromosome abnormalities. |
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| Keywords: | chromosome banding fluorescence in situ hybridization |
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