碱性电解水对D-半乳糖致小鼠血液和肝、肾、脑组织脂质过氧化作用的影响

目的探讨碱性电解水对D-半乳糖致小鼠血液和肝、肾、脑组织脂质过氧化作用的影响。方法将45只成年雄性清洁级昆明小鼠随机分为3组,分别为对照组(皮下注射生理盐水及饮用自来水18周)、D-半乳糖染毒组(皮下注射100mg/kgD-半乳糖,1次/d,同时饮用自来水18周)、D-半乳糖+碱性电解水组(皮下注射100mg/kgD-半乳糖,1次/天,连续18周,并于前6周饮用自来水,后12周饮用碱性电解水),每组15只。于末次暴露后1h,处死动物,取血液、肝脏、肾脏和大脑。采用硫代巴比妥酸法测定脂质过氧化产物丙二醛(MDA)含量。采用DTNB法测定还原型谷胱甘肽(GSH)含量。采用改良DTNB直接法测定谷胱甘肽过氧化物酶(GSH-Px)活力。采用黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)活力。采用Sohal法测定脂褐素含量。结果与对照组相比,D-半乳糖染毒组各组织中MDA、脂褐素含量增加,GSH含量下降,GSH-Px和SOD活力下降;与染毒组相比,D-半乳糖+碱性电解水组各组织中MDA、脂褐素含量下降,GSH含量增加,GSH-Px和SOD活力增加。结论碱性电解水对D-半乳糖所致的脂质过氧化作用有一定的拮抗作用。

Effect of Alkaline Electrolytic Water on Lipid Peroxidation in Blood, Liver, Kidney and Brain of Mouse

Objective To research the protective effects of alkaline electrolytic water on lipid peroxidation in blood,liver, kidney and brain of mice. Methods Total forty-five Kunming mice were randomly divided into three groups,control group:tap water; peroxidation model group: 100 mg/kg D-galaetose subcutaneous injection (once a day) + tap water; experimental group: 100 mg/kg D-galactose subcutaneous injection (once a day) + alkaline electrolytic water. After 18 weeks of exposure,the mice were sacrificed, and the blood,liver, kidney and brain of mice were collected for further study. The MDA levels were measured by TBA method; The GSH levels and GSH-Px activities were measured by modified DNTB method; The SOD activities were measured by Xanthine oxidase method; The lipofuscin levels were detrmined by Sohal method. Results Compared with the control group, MDA and lipofuscin level increased,GSH content decreased,the activities of GSH-Px and SOD decreased in all detected organs and blood in the peroxidation model group, and compared with the peroxidation model group, MDA and lipofuscin level decreased, GSH content increased,the activities of GSH-Px and SOD increased significantly in D-galaetose+alkaline electrolytic water group. Conclusion Alkaline electrolytic water has some antagonistic effects on the lipid peroxidation induced by D-galactose.