原发性高血压与2种相关基因多态性关系

目的 探讨18～45岁人群的原发性高血压（EH）与G蛋白岛亚单位（GNB3）基因825C／T、Y染色体HindⅢ酶切位点多态性的关系。方法 选取原发性高血压患者345例，正常人281名。提取白细胞DNA采用多聚酶链反应结合限制性内切酶（BseDⅠ或HindⅢ）方法检测2种基因的多态性特征。结果 对照组和病例组间GNB3825C／T多态性各基因型和等位基因频率差异无统计学意义（P〉0．05）。Y染色体HindⅢ多态性各基因型差异有统计学意义（P=0．03），EH病人HindⅢ（-）基因型增多。结论 Y染色体HindⅢ酶切位点多态性与青年原发性高血压相关。

Relationship between essential hypertension and two related gene polymorphism

Objective To investigate the relationship of GNB3 825C/T polymorphism and polymorphic HindIII restriction site of the Y chromosome in young aged essential hypertension.Methods 345 patients were enrolled with essential hypertension and 281 healthy subjects as control group.Genomic DNA was extracted from white blood cell.Segments of GNB3 and Y chromosome were amplified from genomic DNA by polymerase chain reaction(PCR).PCR products were subjected to electrophoresis in agarose gels.PCR products were restricted with HindIII or BseDI respectively.The digested products were subjected to electrophoresis in agarose gels and stained with ethidium bromide.Results GNB3 825C/T allele and genotype frequencies did not differ significantly between essential hypertension and control group(P>0.05).The HindIII(+) genotype was found in 55.4% of the men in hypertension and in 67.3% of those in control.The frequency of HindIII(-) genotype was significantly higher than that in the control(P=0.03).Conclusion Polymorphic HindIII restriction site of the Y chromosome maybe is associated with young essential hypertension.