| 重组水蛭素Ⅲ多克隆抗体的制备 |
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| 引用本文： | 张春香,郭丰.重组水蛭素Ⅲ多克隆抗体的制备[J].中国药房,2007,18(34):2658-2660. |
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| 作者姓名： | 张春香 郭丰 |
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| 作者单位： | 1. 南京市江宁医院药剂科,南京市,211000
2. 江苏扬子江药业集团,泰州市,225300 |
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| 摘 要： | 目的:制备重组水蛭素Ⅲ(rHV3)多克隆抗体。方法:分别用戊二醛法与碳二亚胺法处理抗原,电泳检测rHV3自身交联及与牛血清白蛋白(BSA)交联结果;分别免疫家兔、豚鼠和大鼠后取血清,双向免疫扩散试验测定效价。结果:rHV3交联BSA后作为抗原免疫豚鼠和家兔产生了抗体,抗体效价1∶16。结论:rHV3交联BSA作为rHV3抗原免疫家兔、豚鼠可以产生rHV3多克隆抗体。
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| 关 键 词： | 重组水蛭素Ⅲ 免疫 抗体制备 |
| 文章编号： | 1001-0408（2007）34-2658-03 |
| 收稿时间： | 2007-02-07 |
| 修稿时间： | 2007-03-23 |
Preparation of Polyclonal Antibody of Recombinant Hirudin Ⅲ |
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| ZHANG Chunxiang,GUO Feng.Preparation of Polyclonal Antibody of Recombinant Hirudin Ⅲ[J].China Pharmacy,2007,18(34):2658-2660. |
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| Authors: | ZHANG Chunxiang GUO Feng |
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| Institution: | 1.Dept. of Pharmacy, Nanjing Jiangning Hospital, Nanjing 211000, China;2.Yangtze River Pharmaceutical Group, Taizhou 225300, China |
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| Abstract: | OBJECTIVE:To prepare polyclonal antibody for recombinant hirudin Ⅲ (rHV3). METHODS: The antigens were prepared through two typical methods: one was conjugated with rHV3 itself, another was coupled with bovine serum albumin (BSA). Conjugation results were detected through electrophoresis. Then the rabbits, guinea pigs and rats were immunized and with serum sample taken for determination of antibody titer by double immunodiffusion test. RESULTS: Antibodies were produced in rabbits and DHP guinea pigs after immunization using rHV3-BSA as antigen, with antibody titer of 1∶16. CONCLUSION: After being immunized with rHV3-BSA, rabbits and DHP guinea pigs can produce polyclonal antibody for rHV3. |
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| Keywords: | Recombinant hirudin Ⅲ Immunity Preparation of antibody |
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