姜黄素对人多发性骨髓瘤细胞表达Survivin、Bcl-2、Bax的影响

为探讨姜黄素对人多发性骨髓瘤细胞RPMI8226的抑制生长和诱导凋亡的作用机制,应用MTT法检测姜黄素对肿瘤细胞的杀伤效应;用流式细胞术（FCM）分析姜黄素作用后肿瘤细胞凋亡及细胞周期的变化;用RT-PCR方法检测Survivin、Bcl-2、Bax mRNA水平的变化。结果表明：姜黄素明显抑制RPMI8226细胞的增殖,并表现为时间、剂量依赖性,24、48小时IC50值分别为12.15μmol/L、4.9μmol/L;凋亡率由10.6%增加到36.9%（p〈0.05）,细胞发生G2/M期阻滞;RPMI8226细胞细胞强表达Survivin、Bcl-2,弱表达Bax。RPMI8226细胞经10μmol/L姜黄素处理24小时后,survivin、Bcl-2 mRNA表达下调,而Bax表达上调。结论：姜黄素抑制多发性骨髓瘤细胞RPMI8226增殖,并诱导凋亡。姜黄素的抗瘤机制可能与凋亡相关蛋白survivin、bcl-2、bax在转录水平的调节有关。

Effect of Curcumin on Expression of Survivin, Bcl-2 and Bax in Human Multiple Myeloma Cell Line

To explore the mechanisms of suppression growth and induction apoptosis of curcumin on human multiple myeloma cell line RPMI8226, the suppressive effect of curcumin on RPMI8226 was examined by MTT assay; the induction apoptosis and cell cycle arrest of curcumin on RPMI8226 were determined by flow cytometry (FCM); the changes of survivin, Bcl-2, Bax mRNA levels were detected by RT-PCR. The results showed that curcumin obviously suppressed the proliferation of RPMI8226 in both time- and dose-dependent manners, and the IC(50) were 12.15 micromol/L, 4.9 micromol/L for 24 and 48 hours respectively. FCM indicated that the apoptosis ratio rose from 10.6% of untreated cells up to 36.9% of treated cells (p < 0.05), and curcumin arrested cell cycle of RPMI8226 at G(2)/M phase. RT-PCR showed that RPMI8226 cells expressed survivin, Bcl-2 strongly and Bax slightly; while RPMI8226 cells were treated with curcumin 10 micromol/L for 24 hours, the expressions of survivin, Bcl-2 mRNA were apparently down-regulated, and the expression of Bax mRNA was markedly up-regulated. It is concluded that curcumin can suppress the proliferation of human multiple myeloma cell line RPMI8226, and induce their apoptosis. The mechanism of antitumous effect of curcumin may be related to down-regulation of survivin, Bcl-2 mRNA and up-regulation of Bax mRNA.