大小肝癌中相关因子的表达差异及其意义

目的:比较影响大、小肝癌生物学行为差异的相关因素,并探讨其可能的机制。方法:采用免疫组化方法检测nm23-H1、c-Met、MT1-MMP(membrane-type1matrixmetalloproteinase)、Ki-67蛋白在53例大肝癌(瘤体直径>3cm)及40例小肝癌(单肿瘤结节,瘤体直径≤3cm)中的表达,并分析其与肝癌临床病理因子的关系。结果:nm23-H1、c-Met、MT1-MMP蛋白在大、小肝癌组织中的表达差异显著(分别为P=0.005、P=0.008和P=0.007);nm23-H1蛋白表达与肿瘤远处转移之间呈负相关关系(P=0.041);c-Met、MT1-MMP蛋白表达与肿瘤包膜浸润、远处转移之间均呈正相关关系(P<0.05)。结论:大肝癌组织表达nm23-H1、c-Met、MT1-MMP蛋白发生改变,是促使其生物学行为较小肝癌更为恶性的重要因素。

The Different Expression and Significance of the Correlation Factors between Small Hepatocellular Carcinoma and Large Hepatocellular Carcinoma

Objective: To study the factors causing different biological behaviors between SHCC (small hepatocellular carcinoma) and LHCC (large hepatocellular carcinoma) and, to furthermore explore the results of possible mechanisms in these differences. Methods: The expression of nm23-H1, c-Met, MT1-MMP (membrane- type1 matrix metalloproteinase) and Ki-67 protein in 40 cases of SHCC and 53 cases of LHCC were studied by means of immunohistochemistry. Moreover, the relationship between protein expression and clinicopathological factors of hepatocarcinoma was analyzed. Results: The protein expression of nm23-H1, c-Met, MT1-MMP in SHCC differed from that in LHCC remarkably (P=0.005, P=0.008 and P=0.007 respectively). The protein expression of nm23-H1 has a negative correlation with distant metastasis of the tumor (P=0.041). On the other hand, the protein expression of c-Met and MT1-MMP has a positive correlation with tumor invasion and distant metastasis (P<0.05). Conclusion: As a result of alteration of nm23-H1, c-Met, MT1-MMP protein expression in the process of tumor progression, the prognosis of LHCC is poorer than SHCC.