miR-125b和血管内皮生长因子A在肝细胞癌中的表达及两者的相关性

目的:探讨miR-125b与血管内皮生长因子A(vascular endothelial growth factor-A,VEGF-A)在肝细胞肝癌(hepatocellular carcinoma,HCC)中的表达情况及与两者的相关性?方法:利用real-time PCR检测肝癌?癌旁组织?肝癌细胞Huh-7?HepG2以及正常肝细胞L02中miR-125b表达,应用real-time PCR和Western blot分别检测肝癌?癌旁组织中VEGF-A mRNA和蛋白表达?转染miR-125b模拟物(mimic)上调肝癌细胞HepG2中miR-125b表达后,real-time PCR和Western blot检测HepG2细胞中VEGF-A mRNA和蛋白表达?结果:miR-125b 在人肝癌组织中的表达较癌旁组织明显降低(P < 0.05);在肝癌细胞Huh-7?HepG2中的表达较L02明显降低(P < 0.05)?VEGF-A mRNA和蛋白在人肝癌组织中的表达较癌旁组织明显升高(P < 0.05)?miR-125b mimic上调HepG2中miR-125b表达后,转染组VEGF-A mRNA和蛋白表达较空白组和阴性对照组均明显下降(P < 0.05)?结论:miR-125b在肝癌中表达明显下调,而VEGF-A表达上调;低表达的miR-125b丧失对VEGF-A表达的抑制作用可能是肝癌发生的重要机制?

Expression and correlation of miR-125b and vascular endothelial growth factor-A in hepatocellular carcinoma

Objective:To investigate the expression of miR-125b and vascular endothelial growth factor-A (VEGF-A) in hepatocellular carcinoma(HCC) and to analyze their correlation. Methods:Real-time PCR was performed to detect miR-125b expression in HCC,tumor-adjacent tissue and Huh-7,HepG2,L02. Real-time PCR and Western blot were employed to detect the expression of VEGF-A mRNA and protein in HCC,tumor-adjacent tissue. miR-125b mimics was transfected into HepG2 cells and the expression of VEGF-A mRNA and protein were tested with real-time PCR and Western blot,respectively. Results:The expression of miR-125b was down-rengulated in HCC compared to tumor-adjacent tissue(P < 0.05). The expression of miR-125b mRNA in HepG2 cells was also less than to L02 cells(P < 0.05). VEGF-A mRNA and protein were up-regulated HCC and tumor-adjacent tissue compared to normal liver tissue(P < 0.05),which was down-regulated in HepG2 cells after transfection of an miR-125b mimic(P < 0.05). Conclusion:miR-125b is down-regulated,but VEGF-A is up-regulated in HCC,suggesting that the depressed expression of miR-125b promotes the carcinogenesis of hepatocellular carcinoma by upregulating VEGF-A expression.