血浆miRNA表达谱与胰腺癌相关性的研究

目的:探讨血浆miRNA的表达水平与胰腺癌发病的关联?方法:通过TLDA(TaqMan low density array )芯片系统筛选24例胰腺癌患者和24例健康对照间差异表达的miRNA,对差异表达miRNA采用实时荧光定量PCR法进行单个样本验证?结果:miR-451和miR-409-3p的表达水平在胰腺癌组和对照组中有显著的差别,P值分别为0.004 1和0.005 7?这2个miRNAs的组合以93.8%的受试工作者曲线下面积(area under the ROC curve,AUC)将健康对照组和胰腺癌患者组分开(灵敏度为95.8%,特异度为91.7%)?结论:血浆miR-451和miR-409-3p的表达水平可有效区分胰腺癌患者和健康个体?

Correlation of serum microRNA profiling with pancreatic cancer risk

Objective:To investigate the association of the expression level of serum miRNA and the incidence of pancreatic cancer. Methods: Using TaqMan low density array systematically screened the differentially expressed miRNA between the 24 pancreatic cancer patients and the 24 healthy controls. Then those miRNAs that satisfied the criteria were selected for further individual qRT-PCR confirmation. Results: The expression levels of two miRNAs (miR-451 and miR-409-3p) were confirmed to be significantly different in the pancreatic cancer patients and the controls, and P-values were 0.004 1 and 0.005 7,respectively. The area under the ROC curve (AUC) was 0.938 with sensitivity of 95.8% and specificity of 91.7%. Conclusion: The expression level of serum miR-451 and miR-409-3p can successfully distinguish the pancreatic cancer patients from the healthy.