非酒精性脂肪性肝病兔肝组织硫化氢与一氧化氮的变化

目的观察非酒精性脂肪性肝病（NAFLD）兔肝组织硫化氢（H2S）、一氧化氮（NO）浓度,探讨H2S、NO在NAFLD发病中的作用。方法 40只日本大耳白兔随机分为重度NAFLD组（重度组）、轻度NAFLD组（轻度组）、空白对照组（对照组）。重度组给予高脂饲料160 g/（兔.d）,轻度组给予高脂饲料80 g/（兔.d）及普通饲料80 g/（兔.d）,对照组给予普通饲料160 g/（兔.d）。均饲养13 w。实验前后采集血浆标本,检测甘油三酯（TG）、总胆固醇（TC）;肝组织匀浆检测NO、H2S浓度;肝组织HE染色,光镜观察肝脏病理学。结果 （1）饲养后重度组TC、TG分别为（32.12±1.25）、（6.02±2.12）mmol/L,轻度组TC、TG分别为（18.34±2.10）、（4.39±1.93）mmol/L,均高于各组饲养前（P〈0.01或P〈0.05）,饲养后重度组高于轻度组（P〈0.05）。（2）重度组（132.4±20.7）μmol/g蛋白和轻度组（95.4±20.7）μmol/g蛋白肝组织NO浓度显著高于对照组（74.9±34.7）μmol/g蛋白（P〈0.01）,重度组又显著高于轻度组（P〈0.01）。（3）与对照组比较,重度组和轻度组肝组织H2S明显下降（P〈0.01或P〈0.05）,重度组与轻度组比较下降更显著（P〈0.05）。（4）重度组肝脏病理学改变呈重度NAFLD,轻度组呈轻度至中度NAFLD。结论 NO、H2S参与NAFLD的发生、发展,通过干预NO、H2S防治NAFLD可能是未来方向。

Changes of nitrogen oxide and hydrogen sulfide concentration in liver tissues of rabbit with nonalcoholic fatty liver disease

Objective To observe the changes of nitric oxide（NO）and hydrogen sulfide（H2S）concentration in liver tissues of rabbit with nonalcoholic fatty liver disease（NAFLD）,and to discuss the roles of H2S and NO in the pathogenesis of NAFLD.Methods 40 Japanese white rabbits were randomly divided into severe NAFLD group（severe group）,mild NAFLD group（mild group）and blank control group（control group）.Severe group was given high fat diet of 160 g daily per rabbit,mild group were given high fat diet of 80 g plus normal diet of 80 g daily per rabbit,and control group was given normal diet of 160 g daily per rabbit.All animals were fed for 13 w.Plasma samples were collected before and after experiment for the measurement of triglyceride（TG）and total cholesterol（TG）.NO and H2S concentration in liver homogenate were tested.Liver tissues were stained with HE and their pathological changes were observed under light microscope.Results（1） After the feeding of high fat diet,TC level in severe group was higher than that in mild group（32.12±1.25）versus（18.34±2.10）mmol/L,P0.01],so did TG level（6.02±2.12）versus（4.39±1.93）mmol/L,P0.01].In both groups,they were all higher than those before the feeding of high fat diet（P0.01）.（3）NO concentration in liver tissues in severe group（132.4±20.7）μmol/g·pro]increased significantly compared with mild group（95.4±20.7）μmol/g·pro].Both groups showed higher NO concentration than that in control group（74.9±34.7）μmol/g·pro,P0.01].（3）Compared with control group,H2S concentration in liver tissues in severe group and mild group decreased significantly（P0.01）;severe group showed a more significant decrease than mild group（P0.01 or P0.05）.（4）Liver pathologic examination demonstrated severe NAFLD in severe group,and mild to moderate NAFLD in mild group.Conclusion H2S and NO appear to be involved in the occurrence and development of NAFLD.Thus,it is possible to treat NAFLD through the intervention of NO and H2S.