| 鹰嘴豆芽提取物对Caco-2细胞凋亡的作用 |
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| 引用本文： | 毛人杰,窦君,丁剑冰,阿布力米提·伊力,赵海清,程珍,阿吉艾克拜尔·艾萨.鹰嘴豆芽提取物对Caco-2细胞凋亡的作用[J].西南国防医药,2011,21(5):469-472. |
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| 作者姓名： | 毛人杰 窦君 丁剑冰 阿布力米提·伊力 赵海清 程珍 阿吉艾克拜尔·艾萨 |
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| 作者单位： | 1. 新疆医科大学基础医学院免疫学教研室,乌鲁木齐,830054
2. 中国科学院新疆理化技术研究所新疆植物资源化学重点实验室 |
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| 基金项目： | 中国科学院、国家外国专家局创新团队国际合作伙伴计划;"973"计划前期研究专项，新疆维吾尔自治区科技支疆项目 |
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| 摘 要： | 目的研究鹰嘴豆芽乙醇提取物（CSE）对人结肠腺癌Caco-2细胞增殖及细胞凋亡的影响。方法提取鹰嘴豆芽的活性成分并测定含量;MTT法观察提取物对Caco-2细胞增殖的影响;透射电镜观察细胞超微结构;琼脂糖凝胶电泳法进行细胞凋亡的DNA分析;流式细胞术检测凋亡情况。结果 CSE含23.53%皂苷、14.73%异黄酮;不同浓度的CSE对Caco-2细胞均有一定的增殖抑制作用,且呈量效和时效关系;细胞出现染色质边移和凋亡小体;DNA电泳呈现梯度条带,表明CSE可诱导Caco-2细胞凋亡;3、5、10μg/ml的CSE作用后,细胞凋亡率分别为32.6%、68.8%、73.9%,与对照组（0.9%）比较差异显著（P〈0.05）。结论 CSE可通过诱导细胞凋亡而抑制人结肠腺癌株Caco-2细胞的生长,为鹰嘴豆应用于结肠癌的临床治疗和预防提供了理论依据。
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| 关 键 词： | 鹰嘴豆 Caco-2细胞 细胞凋亡 |
Effects of chickpea sprout extract on apoptosis of Caco-2 cells in vitro |
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| Mao Renjie,Dou Jun,Ding Jianbing,YILI Ablimiti,Zhao Haiqing,Cheng Zhen,AISA Haji Akber.Effects of chickpea sprout extract on apoptosis of Caco-2 cells in vitro[J].Medical Journal of National Defending forces in Southwest China,2011,21(5):469-472. |
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| Authors: | Mao Renjie Dou Jun Ding Jianbing YILI Ablimiti Zhao Haiqing Cheng Zhen AISA Haji Akber |
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| Institution: | Mao Renjie1,2,Dou Jun2,Ding Jianbing1,YILI Ablimiti2,Zhao Haiqing2,Cheng Zhen2,AISA Haji Akber2 1.Department of Immunology,Xinjiang Medical University,Urumqi,Xinjiang,830054,China,2.Xinjiang Key Laboratory of Plant Resources and Natural Products Chemistry,Xinjiang Techonological Institute of Physics and Chemistry,Chinese Acadamy of Sciences |
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| Abstract: | Objective To explore the effects of chickpea sprout ethanol extract(CSE)on the proliferation and apoptosis of human colon adenocarcinoma cell line Caco-2 in vitro.Methods The active elements of chickpea sprout-CSE,were extracted and its components were analyzed.The proliferation inhibition of CSE on Caco-2 cells was measured by MTT assay.The ultrastructure of these cells was observed under electron microscope.Apoptosis was assayed by agarose gel electrophoresis,and cell apoptosis rate was evaluated by flow ... |
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| Keywords: | chickpea Caco-2 cell apoptosis |
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