结晶型NiS诱发人支气管上皮细胞系恶性转化

目的:研究结晶型硫化镍(NiS)诱发SV-40 Large T抗原永生化的人支气管上皮细胞系(16HBE)恶性转化作用.方法:体外用不同浓度结晶型NiS多次处理16HBE细胞,软琼脂集落形成和裸鼠成瘤试验鉴定转化细胞的恶性度.结果:细胞培养至35代,发现NiS可诱导16HBE恶性转化.转化细胞排列紊乱,重叠生长,在软琼脂中的集落形成率显著高于对照组(P＜0.01),并呈时间剂量依赖关系;转化细胞在裸鼠体内成瘤,组织学证实为低分化鳞状细胞癌.结论:结晶型NiS有较强的诱导人支气管上皮细胞恶性转化能力；该转化模型为镍致癌机制的分子水平研究提供了理想的研究系统。

MALIGNANT TRANSFORMATION OF HUMAN BRONCHIAL EPITHELIAL CELL LINE INDUCED BY CRYSTALLINK NICKEL SULFIDE

Purpose: To study the malignant transforming activity of crystalline nickel sulfide (NiS) in human bronchial epithelial cell line (16HBE) immortalized by SV-{40} Large T antigen. Methods: 16HBE cells were trea-{ted several} times with NiS at different concentrations in vitro, and the malignant degree of transformed cells were identified by the assay for anchorage-independent growth and tumorigenicity. Results: The 35+{th} passage cells exhibited overlapping growth. As compared with that of negative control cells, colony formation efficiency of the transformed cells in soft agar showed significant increases (P<0.01), and a dose-dependent effect. The transformed cells could grow into tumor in nude mice, a squamous cell carcinoma confirmed by histopathological examination. Conclusion: Crystalline nickel sulfide can induce malignant transformation of 16HBE cells. This transformed model provides a potential tool for the study on molecular mechanism of nickel carcinogenesis.