| 中国脑膜炎奈瑟菌penA基因及PBP2蛋白多态性研究 |
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| 引用本文： | 李马超,郑玉红,徐丽,任红宇,高源,朱兵清,田国忠,邵祝军. 中国脑膜炎奈瑟菌penA基因及PBP2蛋白多态性研究[J]. 疾病监测, 2010, 25(2): 130-133. DOI: 10.3784/j.issn.1003-9961.2010.02.014 |
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| 作者姓名： | 李马超 郑玉红 徐丽 任红宇 高源 朱兵清 田国忠 邵祝军 |
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| 作者单位： | 1.中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京 102206 |
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| 摘 要： | 目的掌握中国脑膜炎奈瑟菌对青霉素类抗生素敏感性的变化情况,探讨脑膜炎奈瑟菌菌株对青霉素敏感性降低的机制、敏感性变化与penA基因及PBP2蛋白多态性之间的关系。方法采用MIC肉汤稀释法和E-test药敏纸条法检测脑膜炎奈瑟菌菌株对青霉素的敏感性,并对penA基因进行扩增、测序,分析penA以及PBP2蛋白与菌株的青霉素敏感性变化的关系。结果通过肉汤稀释法和E-test法检测出4株对青霉素中介敏感菌株,未发现对青霉素耐药菌株;菌株的药物敏感性变化情况与penA及PBP2蛋白的多态性无明显关系,所检测菌株的PBP2高度保守。结论应该继续对流脑菌的青霉素敏感性变化情况开展基因水平监测,及时发现高耐药性菌株,防止耐药菌株克隆在人群间扩散。
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| 关 键 词： | 脑膜炎奈瑟菌 敏感性 penA测序 |
| 收稿时间： | 2009-06-15 |
Study of the polymorphisms of Neisseria meningitidis penA gene and PBP2 protein in China |
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| LI Ma-chao,ZHENG Yu-hong,XU Li,REN Hong-yu,GAO Yuan,ZHU Bing-qing,TIAN Guo-zhong,SHAO Zhu-jun.State Key Laboratory for Infectious Disease Prevention , Control,National Institute for Communicable Disease Control , Prevention,Chinese Center for Disease Control , Prevention,Beijing ,China. Study of the polymorphisms of Neisseria meningitidis penA gene and PBP2 protein in China[J]. Disease Surveillance, 2010, 25(2): 130-133. DOI: 10.3784/j.issn.1003-9961.2010.02.014 |
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| Authors: | LI Ma-chao ZHENG Yu-hong XU Li REN Hong-yu GAO Yuan ZHU Bing-qing TIAN Guo-zhong SHAO Zhu-jun.State Key Laboratory for Infectious Disease Prevention Control National Institute for Communicable Disease Control Prevention Chinese Center for Disease Control Prevention Beijing China |
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| Affiliation: | 1.State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206, China |
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| Abstract: | Objective To disclose the changes in the susceptibility of Neisseria meningitidis to penicillin antibiotics in China,and explore the mechanism for reduction of the penicillin susceptibility of Neisseria meningitidis strains and the relationship between the susceptibility changes and the polymorphisms of penA gene and PBP2 protein.Methods Both MIC broth microdilution and E-test methods were employed to determine the susceptibility of Neisseria meningitidis strains to penicillin.Amplification and sequencing o... |
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| Keywords: | Neisseria meningitidis susceptibility penA sequencing |
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