| 分化早期胚胎大鼠神经干细胞电生理特征 |
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| 引用本文： | 冷洁,蒋莉,陈恒胜.分化早期胚胎大鼠神经干细胞电生理特征[J].第三军医大学学报,2010,32(3). |
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| 作者姓名： | 冷洁 蒋莉 陈恒胜 |
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| 作者单位： | 重庆医科大学儿童医院神经内科,重庆,400014 |
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| 基金项目： | 国家自然科学基金(30672217);;重庆市教委科研基金(06-2-158)~~ |
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| 摘 要： | 目的探索分化早期神经干细胞的电生理特征。方法分离培养胚胎大鼠海马神经干细胞。应用膜片钳全细胞记录技术检测神经干细胞和分化早期(1～3 d)神经细胞的电压门控离子电流。结果未分化的神经干细胞不表达内向钠离子电流,而诱导分化1 d的神经细胞即表达内向性钠离子电流,但不能诱导产生动作电位。神经干细胞和分化早期的神经细胞均表达两种类型的外向钾离子电流,并表达内向T-型钙电流。结论钠离子电流的功能性表达是神经干细胞退出细胞周期开始分化的标志。分化早期是神经干细胞离子通道发育过程的重要阶段。
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| 关 键 词： | 膜片钳 海马 神经干细胞 分化 离子通道 |
Electrophysiological characteristics of early-differentiated neural stem cells in embryonic rats |
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| Leng Jie,Jiang Li,Cheng Hengsheng.Electrophysiological characteristics of early-differentiated neural stem cells in embryonic rats[J].Acta Academiae Medicinae Militaris Tertiae,2010,32(3). |
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| Authors: | Leng Jie Jiang Li Cheng Hengsheng |
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| Institution: | Department of Neurology;Children's Hospital;Chongqing Medical University;Chongqing;400014;China |
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| Abstract: | Objective To study the electrophysiological characteristics of early-differentiated neural stem cells(NSC)in embryonic rats.Methods NSC were isolated from hippocampus of embryonic rats and cultured.The cells were plated onto poly-llysine hydrochloride glass cover-slips for 1 h or differentiated for 1-3 d in a differentiation medium.Whole cell patch-clamp technique was used to record the voltage-gated ion channel current.Results No inward Na+ current was detected in any undifferentiated NSC.One day after ind... |
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| Keywords: | patch-clamp technique hippocampus neural stem cell differentiation ion channel |
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