异种(猪)无细胞真皮基质与自体微粒皮复合移植修复深度烧伤创面的实验研究

目的　观察异种 (猪 )无细胞真皮支架与自体微粒皮加同种异体皮复合移植修复全层皮肤缺损的效果。方法　 4 8只 SD大鼠背部全层皮肤缺损创面 ,分别行异种 (猪 )无细胞真皮支架与自体微粒皮移植(复合皮组 )和单纯微粒皮移植 (对照组 ) ,术后定期观察创面愈合情况并行创面愈合率和收缩率的计算 ,同时取创面组织进行组织学观察。结果　复合皮组的创面愈合情况良好 ,未见明显挛缩 ,皮肤弹性较好 ,两组异体皮成活情况及创面愈合情况相近 ,但是复合皮组创面收缩率显著低于对照组。组织学观察复合皮组上皮化良好 ,胶原纤维排列有序 ,基底膜结构完整。结论　异种 (猪 )无细胞真皮支架与自体微粒皮复合移植能修复全层皮肤缺损 ,改善创面愈合质量。

Experimental study on composite transplantation of acellular porcine dermal matrix and micro-autograft to repair deep burn wound

Objective To observe the effect of composite skin graft consisting of heterogeneous(swine) acellular dermal matrix and combined micro-autograft and allograft with the aim at overcoming shortcomings of repairing materials for burn wound. Methods Forty-eight Sprague-Dawley rats with full-thickness skin defect on the back were grafted with the composite skin(compound skin grafting group, G)and autologous particulate skin(control group, C), respectively. The area of wound healing and the rates of wound contraction were measured. Tissue specimens were harvested for histological examination. Results The composite skin graft resulted in less contracture and better elasticity. Compared with group C, the wound contraction rate was lower significantly. Histological examination revealed that there was full differentiation of epithelium, orderly collagen arrangement and intact basement membrane. Conclusion Coverage of wound with composite skin composing heterogeneous(swine) acellular dermal matrix and autologous particulate skin could promote wound healing and improve wound healing quality.