乳腺癌中P16及P21基因的初步研究

目的 :初步探测 P16及 P2 1基因异常在乳腺癌发生发展中的作用。方法 :采用多重 PCR扩增 P16及 P2 1基因外显子 2 ,免疫组化染色 (SP法 )检测 P16及 P2 1蛋白表达。结果 :本组乳腺癌中 P16基因外显子 2的纯合子缺失频率为47.2 % (17/ 36 ) ,蛋白阴性占 19.4% (7/ 36 ) ;未检出 P2 1基因外显子 2纯合子缺失 ,而蛋白阴性占 36 .1% (13/ 36 )。三种类型的乳腺癌中 P16及 P2 1基因异常频率有明显差异 ,淋巴转移组的基因异常频率极显著高于未转移组 (P<0 .0 1)。两种基因同时异常占 13.9% (5 / 36 )。结论 :P16及 P2 1基因异常与乳腺癌的病理组织分型相关 ,与乳腺癌的恶性进程密切相关。

Deletions of P16 Gene and P21 Gene in Human Breast Carcinomas.

Objective:To study the role of deletions of P16 and P21 genes in human breast carcinoma . Methods:P16 and P21 gene exon2 were detected with Mutiplex PCR method.Both P16 and P21 protein expressions were detected by SP immunohistochemistry technique. Results:The frequency of P16 gene exon2 deletion was 47.2%(17/36).The negative rate of P16 protein was 19.4%(7/36).There was no any deletion of P21 gene in the detection,but the negative rate of P21 protein was 36.1%(13/36). Deletions of P16 and P21 gene among three types of breast carcinoma were significantly different,and also there was a significant difference in that between metastatic and non metastatic breast carcinoma to lymph node( P <0.01).Conclution: Deletions of P16 and P21 gene are related to the histology type of human breast carcinomas,and closely related to the progression of human breast carcinoma.