| 尼卡地平对结肠癌细胞HCT116/L耐药的逆转作用研究 |
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| 引用本文： | 冯森玲,梁宝莹,谭湘萍,梅峥嵘,袁中文. 尼卡地平对结肠癌细胞HCT116/L耐药的逆转作用研究[J]. 今日药学, 2021, 31(2): 110-114 |
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| 作者姓名： | 冯森玲 梁宝莹 谭湘萍 梅峥嵘 袁中文 |
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| 作者单位： | 广州医科大学附属第三医院药学部,广东省产科重大疾病重点实验室,广东广州510150;广州中医药大学第二附属医院,广东省中医院皮肤科,广东广州510120 |
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| 基金项目： | 广东省基础与应用基础研究基金区域联合基金(2019A1515111109);广东省医院药学研究基金(2020A28);广州医科大学附属第三医院2019年度博士启动科研项目(2019B03);广东省医学科学技术研究基金项目(A2020365);广东省中医药局科研项目(20201213)。 |
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| 摘 要： | 目的 探究尼卡地平逆转耐奥沙利铂结肠癌细胞HCT116/L耐药的疗效及其机制.方法 采用MTT法分别检测尼卡地平和奥沙利铂在HCT116/L和HCT116细胞上的药敏性,并检测尼卡地平能否逆转奥沙利铂耐药.采用流式细胞仪(FCM)检测HCT116/L细胞凋亡和细胞内罗丹明-123的浓度.结果 奥沙利铂在耐药细胞HCT1...
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| 关 键 词： | 尼卡地平 奥沙利铂 人结肠癌细胞 耐药 |
The Reversal Effect of Nicardipine on HCT116/L Colon Cancer Cells |
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| FENG Senling,LIANG Baoying,TAN Xiangping,MEI Zhengrong,YUAN Zhongwen. The Reversal Effect of Nicardipine on HCT116/L Colon Cancer Cells[J]. Pharmacy Today, 2021, 31(2): 110-114 |
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| Authors: | FENG Senling LIANG Baoying TAN Xiangping MEI Zhengrong YUAN Zhongwen |
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| Affiliation: | (Department of Pharmacy,Key Laboratory for Major Obstetric Diseases of Cungdong Prowince,the Third Affiliated Hospital of Guangzhou Medical University,Guangzhou,Guangdong 510150,China;Department of Dermatology,the Second Afiliated Hospital of Guangzhou Unitrersity of Traditional Chinese Medlicine,Guangdong Proincial Hospital of Traditional Chinese Medlicine,Guangzhou,Guangdong 510120,China) |
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| Abstract: | OBJECTIVE To study the mechanism of nicardipine reverse cell resistance in HCT116/L cells,and to detect the effect of combination of nicardipine and oxaliplatin on HCT116/L cells.METHODS MTT method was used to detect the drug sensitivity of nicardipine and oxaliplatin on HCT116/L and HCT116 cells,and to detect whether nicardipine could reverse oxaliplatin resistance.Flow cytometry(FCM)was used to detect the apoptosis of HCT116/L cells and the content of Rho123 in HCT116/L cells.RESULTS The IC50 of oxaliplatin in drug-resistant cells HCT116/L and parental cells HCT116 were(20.07±1.32)μM and(0.65±0.05)μM,respectively,showing significant differences in drug resistance(P<0.001).And the resistance index(RI)was 30.86±2.21.The IC50 of nicardipine in HCT116/L and HCT116 were(22.76±1.52)μM and(21.65±1.35)μM,respectively.There was no statistical difference in cytotoxicity between the two cells.FCM assay showed that nicardipine combining with oxaliplatin could significantly increase the apoptosis rate of HCT116/L(P<0.001),and significantly increase the content of Rho123 in drug-resistant cells HCT116/L.CONCLUSION Nicardipine can reverse the resistance of HCT116/L cells to oxaliplatin.The effect of reversing resistance are related to induce the apoptosis of HCT116/L cells and affect the function of the P-gp transport protein. |
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| Keywords: | nicardipine oxaliplatin HCT116/L drug resistance |
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