离子凝胶法制备壳聚糖纳米粒的影响因素研究

目的探讨离子凝胶法制备壳聚糖纳米粒(CS-NPs)的影响因素。方法用碱降解法制备高脱乙酰度的壳聚糖(CS),并以之为材料,采用离子凝胶法制备CS-NPs,以微粒的平均粒径、分散度和Zeta电位为指标,考察CS及三聚磷酸钠(TPP)的质量浓度、CS/TPP质量比、CS溶液p H值和CS溶液温度对制备CS-NPs的影响。并用透射电子显微镜观察纳米粒的形态。结果CS-NPs的平均粒径随CS、TPP质量浓度及CS/TPP质量比的增大而增大,CS/TPP质量比的增加、CS溶液p H值的降低可引起CS-NPs的Zeta电位增高,CS溶液p H值和温度对CS-NPs的分散度影响较大;制备的壳聚糖纳米粒形态较为规则,类似球形。结论CS及TPP的质量浓度、质量比、CS溶液p H值是制备壳聚糖纳米粒并影响其特征的主要因素。

Study on Influencing Factors for Preparation of Chitosan Nanoparticles by Ionic Gelation Technique

OBJECTIVE To explore the factors for the preparation of chitosan nanoparticles(CS-NPs)by ionic gelation technique.METHODS Highly deacetylated chitosan(CS)were prepared by alkaline degradation,land were used as materials to prepare CS-NPs by ionic gelation technique.Variations in CS mass concentration,sodium tripolyphosphate(TPP)mass concentration,CS to TPP mass ratio,pH value and temperature of CS solution were examined systematically for their effects on the perparatiopn of CS-NPs by using nanoparticle size,dispersion and zeta potential as indexes.Besides,the morphology of nanoparticle was observed by transmission electron microscope.RESULTS The nanoparticle average size was increased by increasing of CS mass concentration,TPP mass concentration and CS/TPP mass ratio.When CS to TPP mass ratio increased and pH value of CS solution decreased,zeta potential increased.The pH value and temperature of CS solution had great influence on the dispersion of CS-NPs.Chitosan nanoparticles had a regular shape quite like a sphere.CONCLUSION CS mass concentration,TPP mass concentration,CS/TPP mass ratio and p H value of CS are the main factors influencing the preparation and characteristics of CS-NPs.