Bcl—2和Fas基因在瘢痕成纤维细胞中的表达

目的研究凋亡基因Fas/Apo-1和抑凋亡相关基因Bcl-2在瘢痕形成机制中的作用.方法采用免疫组织化学方法对10例正常皮肤、10例增生性瘢痕及10例瘢痕疙瘩成纤维细胞Fas/Apo-1和Bcl-2蛋白的表达进行检测.Fas蛋白稀释为1∶50;Bcl-2蛋白稀释为1∶40,阴性对照以PBS代替一抗.随机选择五个高倍视野,计算其细胞平均阳性率.结果Bcl-2蛋白在正常皮肤、增生性瘢痕及瘢痕疙瘩中阳性率分别为6.78%、38.6%和83.2%.瘢痕疙瘩、增生性瘢痕的表达阳性率高于正常皮肤,有非常显著性差异(P＜0.01),而瘢痕疙瘩的表达阳性率明显高于增生性瘢痕(P＜0.01).Fas/Apo-1蛋白在正常皮肤、增生性瘢痕及瘢痕疙瘩中阳性率分别为78.4%、80.4%和84.4%,三组间无显著性差异(P＞0.05).结论病理性瘢痕的形成与Bcl-2基因的过度表达有关,而对Fas基因介导的凋亡不敏感或凋亡受阻,亦是导致瘢痕过度增生原因之一.

EXPERIMENTAL STUDY OF Bcl-2 AND Fas GENE EXPRESSION IN FIBROBLAST OF SCAR

Objective To explore the effect of Fas/Apo-1 and Bcl-2 gene e xpression on mechanism of scar formation. Methods Immunohi stochemical method was applied to defect the expression of Fas and Bcl-2 protein in fibroblasts f r om 10 cases with normal skin, 10 cases with hypertrophic scar and 10 cases with keloid. Results The positive expression rate of Bcl-2 pro tein in keloid was 83.2%, significantly higher than that in hypertrophic scar(38.6%),( P< 0.01), and the positive expression rate in hypertrophic scar and keloid was higher tha n that in normal skin (6.78%), (P< 0.01). But the positive expression rate of Fas/Apo-1 p rotein was 78.4% in normal skin 80.4% in hypertrophic scar, 84.4% in keloid re spectively, which showed no significant difference among them ( P> 0.05). Conclusion Bcl-2 gene but Fas gene may take part in the formation of pathologic sca r.