HPLC测定半边莲药材中2个多炔类成分的含量

目的:建立测定半边莲药材中2个多炔类成分lobetyolin和lobetyolinin的HPLC定量分析方法。方法:采用C₁₈柱,乙腈-水(0～25 min,10%~40%乙腈)梯度洗脱,流速1.0 mL.min^-1,检测波长267 nm。结果:半边莲药材中2个多炔类成分含量普遍较低,其中最高者含lobetyolin和lobetyolinin分别为0.461,0.436 mg.g^-1,总量接近1‰,而在有的样品中未检测到。结论:本方法简便可行,适用于半边莲药材中lobetyolin和lobetyolinin的含量测定。

Determination of two polyacetylenes in Herba Lobeliae Chinensis by HPLC analysis

Objective: To develop an HPLC method for determination of two polyacetylenes,lobetyolin and lobetyolinin,in Herba Lobeliae Chinensis.Method: C₁₈ column was used with the mobile phase consisted of acetonitrile and water.Linear gradient elution from 10% to 40% acetonitrile in 25 min was applied,at the flow rate of 1.0 mL·min^-1,the detection wavelength was at 267 nm.Result: Lower contents of lobetyolin and lobtyolinin were found in collected samples of Herba Lobeliae Chinensis.The highest amounts of lobetyolin and lobetyolinin were found to be 0.461 and 0.436 mg·g^-1 in a sample procured from Hong Kong.However,there were no lobetyolin and lobetyolinin in some of the samples.Conclusion: A simple and effective HPLC method to analyze the two polyacetylenes in Herba Lobeliae Chinensis was established.It could be applied for the quality control of this herb.