A novel erythromycin, 6-desmethyl erythromycin D,made by substituting an acyltransferase domain of the erythromycin polyketide synthase |
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Authors: | Petkovic Hrvoje Lill Rachel E Sheridan Rose M Wilkinson Barrie McCormick Ellen L McArthur Hamish A I Staunton James Leadlay Peter F Kendrew Steven G |
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Affiliation: | Biotica Technology Limited, 181 A Huntingdon Road, Cambridge CB3 0DJ, UK. |
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Abstract: | The acyltransferase (AT) domain in module 4 of the erythromycin polyketide synthase (PKS) was substituted with an AT domain from the rapamycin PKS module 2 in order to alter the substrate specificity from methylmalonyl-CoA to malonyl-CoA. The resulting strain produced 6-desmethyl erythromycin D as the predominant product. This AT domain swap completes the library of malonyl-CoA AT swaps on the erythromycin PKS and reinforces PKS engineering as a robust and generic tool. |
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