| 太原地区妇女儿童淋球菌感染调查及基因快速诊断 |
| |
| 引用本文： | 李连青,朱庆义,贺润花,刘建萍,赵瑞珍.太原地区妇女儿童淋球菌感染调查及基因快速诊断[J].中华流行病学杂志,1997,18(2):98-101. |
| |
| 作者姓名： | 李连青 朱庆义 贺润花 刘建萍 赵瑞珍 |
| |
| 作者单位： | 山西省妇幼保健院儿童医院分子微生物研究室!太原，030013，山西省妇幼保健院儿童医院分子微生物研究室!太原，030013，山西省妇幼保健院儿童医院分子微生物研究室!太原，030013，山西省妇幼保健院儿童医院分子微生物研究室!太原，030013，山西省妇幼保健院儿童医院分子微生物研究室!太 |
| |
| 摘 要： | 改进并建立快速检测淋球菌基因的聚合酶链反应，同时用涂片染色筛查和培养法作鉴定比较，对太原地区１９８６￣１９９５年妇女儿童淋球菌感染状况作调查分析，对９２０４例妇女儿童生殖道感染者作直接涂片染色检菌，阳性率为２６．５％，其中１２２０例同时用培养与ＰＣＲ法检测，阳性率为１２．４％和２１．０％，用ＰＣＲ直接检测临床标本ＮＧ－ＤＮＡ，具有简便，快速、敏感特异之优点。
|
| 关 键 词： | 聚合酶链反应 诊断 淋病 基因诊断 妇女 儿童 |
| 收稿时间： | 1996/7/20 0:00:00 |
| 修稿时间： | 1996/8/24 0:00:00 |
Using Polymerase Chain Reaction for the Detection of Gonorrhoea in Women and Children in Taiyuan |
| |
| Li Lian-qing,Zhu Qing-yi,He Run-hua.Using Polymerase Chain Reaction for the Detection of Gonorrhoea in Women and Children in Taiyuan[J].Chinese Journal of Epidemiology,1997,18(2):98-101. |
| |
| Authors: | Li Lian-qing Zhu Qing-yi He Run-hua |
| |
| Institution: | Department of Molecular Microbiology, Shanxi Maternal and Children''s Hospital, Taiyuan 030013 |
| |
| Abstract: | A polymerase chain reaction (PCR) method was established for the detection of gonococcal gene in samples obtained from women and children with lower genital tract infection. The primers were used for PCR amplification derived from sequence for DNA representing part of the open reading frame (ORF) for the chromosome of N.gonorrhoea.In this study,a total number of 9204specimens were obtained from women and children with lower genital tract infection from 1986 to 1995in Taiyuan. The positive rate of N. gonorrhoea for Gram's stain of smears was 26. 5 % while the rates for culture and PCR were 12. 4% and 21.0%, respectively. The results showed that PCR test was simple,rapid sensitive and specific for the detection of gonococcal gene in clinical specimens. |
| |
| Keywords: | Polymerase chain reaction Rapid diagnosis Gonorrhoea |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《中华流行病学杂志》浏览原始摘要信息 |
| 点击此处可从《中华流行病学杂志》下载免费的PDF全文 |
