Survivin蛋白在肺腺癌中的表达及临床意义

目的探讨Survivin蛋白在肺腺癌组织中的表达及其与临床病理特征的关系。方法用链霉菌生物素蛋白-过氧化物酶免疫组织化学法(SP法),检测28例手术切除的肺腺癌组织、20例正常肺组织中Survivin蛋白的表达;并用末端转移酶标记(TUNEL)法检测凋亡指数(AI)。结果53.6%的肺腺癌组织有Survivin蛋白表达,20例正常肺组织中无Survivin蛋白表达(P<0.001)。Survivin蛋白与肺腺癌组织病理分级、TNM分期、淋巴结转移、预后、p53蛋白过表达呈正相关,而与性别、年龄、吸烟史无明显相关性(P均大于0.05)。AI显示出了Survivin蛋白的抗凋亡作用。结论Survivin蛋白在肺腺癌组织发生发展中起抑制癌细胞凋亡的重要作用,从而Survivin基因有望成为基因治疗的新靶点。

The expression of survivin in lung adenocardinoma and its clinicae significance

Objective To study the expression of survivin at protein level in Lung adnocancinema and its relationship with clinic pathological features.Methods Survivin protein expression was determined in 28 cases of lung adenocar cinoma tissues and 20 cases of normal lung tissues by streptavidin-peroxidase immunohistochemical technique.Apoptotic index(AI) were determined by TUNEL.Results Expression of survivin protein was detected in 15 cases((53.6%)) of lung adenocarcinoma tissues,but no expression of survivin protein in 20 cases normal lung tissues was found(P<(0.001)).Statistically,there was no relationship between survivin protein and sex,age,smoking history.A correlation was found between survivin exprexsion and pathological grades,TNM stages,lymph node metastases,prognosis.AI showed that survivin protein has the anti-apotosis role.Conclusions Expression of Survivin in adenocarcinoma of lung suggested that the protein may play a important role in the pathway of carcinogenesis and the Survivin gene may be potential therapeutic target in lung adenocarcinoma cancer.