血清高速泳动族蛋白B1水平与糖尿病患者急性冠状动脉综合征关系的研究

目的:探讨血清高速泳动族蛋白B1(HMGB1)水平与2型糖尿病(DM)患者急性冠状动脉综合征(ACS)发生的关系。方法:选取218例ACS患者,其中不稳定型心绞痛(UAP)95例,急性心肌梗死(AMI)123例。健康体检者234名作为对照;采用酶联免疫吸附法(ELISA)检测血清HMGB1、高敏C反应蛋白(hsCRP)和肿瘤坏死因子α(TNF-α)的水平。并对血清HMGB1水平与TNF-α、hsCRP和血糖水平进行相关性分析。结果:与对照组相比,AMI组、UAP组血清HMGB1[(12.70±6.72)μg/L、(7.68±3.63)μg/L比(3.83±1.72)μg/L,P<0.01]水平均明显升高。在对照组、UAP组和AMI组的亚组中,DM患者血清HMGB1水平均高于非DM者(均P<0.05)。血清HMGB1水平与hsCRP,TNF-α、血糖水平均呈正相关(均P<0.05)。结论:ACS患者HMGB1水平显著升高,且合并DM的ACS患者HMGB1水平高于非DM患者,表明HMGB1有可能参与ACS的发生、发展过程,且在糖尿病时对ACS的病理生理过程影响更大。

Correlation between level of serum high mobility group box-1 and acute coronary syndrome in patients with type 2 diabetes mellitus

Objective To investigate the correlation between serum high mobility group box-1 （HMGB1） level and acute coronary syndrome （ACS） in patients with type 2 diabetes mellitus（DM）. Methods The study consisted of 234 non coronary artery disease patients （control group） and 218 patients with ACS （ACS group）, which included 95 patients with unstable angina pectoris （UAP） and 123 patients with acute myocardial infarction （AMI）. Two hundred and thirty-four healthy subjects served as controls. Serum levels of HMGB1, high sensitivity C-reactive protein （hsCRP） and tumor necrosis factor （TNF）-α were measured using commercially available enzyme-linked immunosorbent assay （ELISA） kits. Results Serum HMGB1 level was significantly elevated in AMI and UAP groups as compared with controls [（12.70±6.72） μg/L, （7.68±3.63） μg/L vs. （3.83±1.72）μg/L, P〈0.01）]; levels of hsCRP[（3.30±2.97） mg/L, （1.46±1.37） mg/L vs. （0.83±0.69） mg/L, P〈0.05] and TNF-α [（71.04±38.34） ng/L, （52.69±24.76） ng/L vs. （43.27±20.56） ng/L, P〈0.01] were also significantly increased in AMI and UAP groups when compared with those of eontrols. Moreover, serum HMGB1 levels in patients of eaeh group with DM were higher than the counterparts without DM （all P/〈0.05）. HMGB1 level was closely correlated with hsCRP, TNF-α and fasting plasma glucose （FG） levels. Conclusions Serum HMGB1 Ievel was inereased in patients with ACS, and HMGB1 level was higher in patients with DM than those without DM. These results indicated that HMGB1 might be involved in the development and progress of ACS, and the impact was more prominent in the presence of DM.