桂枝芍药知母汤对不同时间点大鼠急性足趾肿胀及炎症介质的影响

目的：建立角叉菜胶诱导的大鼠足趾肿胀模型，观察桂枝芍药知母汤对足趾肿胀的时序性影响及不同起效时间点炎症介质的影响。方法：在大鼠左后肢足趾皮下注射入一角叉菜胶0．1mL/只致炎，随后于0、1、2、3、4、5、6、7、8、9、10h各测一次足趾容积，计算足趾肿胀度和肿胀抑制率，确定最佳起效时间范围；同时研究桂枝芍药知母汤在起效时间内不同时间点对炎症介质的影响。结果：桂枝芍药知母汤高（20g/kg）、中剂量（10g/kg）在1、2、3h，而低剂量组（5g/kg）在3h都能明显降低足趾肿胀度和提高肿胀抑制率；与模型组比较，桂枝芍药知母汤组在1h时血清中COX-2，PGE2，TNF-α以及NO均明显降低（P〈0．05），在3h时血清中IL-1β（P〈0．01）、MPO（P〈0．05）、TNF-α（P〈0．01）、PGE2（P〈0．01）的分泌显著降低。结论：桂枝芍药知母汤抗角叉菜胶致急性炎症作用的有效时间范围是1-3h，在1h主要抑制花生四烯酸的COX-2途径，减少了PGE2、NO和TNF-α的释放，在3小时则主要抑制IL-113．TNF-α、PGE2及MPO等炎症介质的释放，体现了中药复方桂枝芍药知母汤抗炎作用的时序变化特点。

Effect of Guizhi Shaoyao Zhimu Decoction on Acute Swelling and its Inflammatory Mediators at Different Time Points

Objective ： To study Guizhi Shaoyao Zhimu Decoction on anti - inflammatory effect and inflammatory mediators release by es tablishing acute inflammatory model induced by λ - carrageenan. Method： After injecting toe carrageenan 0. 1 ml into each rat left hind toe subcutaneously, toe volume was measured at 1,2,3,4,5,6,7,8,9, lOh, then toe swelling degree and change of inflammatory mediators were determined at different time points. Results： The high and medium dose Guizhi Shaoyao Zhimu Decoction could signifi cantly reduce the swelling degree and improve the swelling inhibition rate at 1,2,3 hours, but the low dose medicine had obvious effect at 3 hour; Compared with the model group, serum COX - 2, PGE2, TNF - α and NO on Guizhi Shaoyao Zhimu Decoction group were obviously decreased at 1 hour （P 〈 O. 05 ）, while serum IL - 1 （ P 〈 0.01 ）, MPO （ P 〈 0.05 ）, TNF -α （ P 〈 O. O1 ）, PGE2 （ P 〈 0. 01 ） decreased at 3hour. Conclusion： The therapeutic time of Guizhi Shaoyao Zhimu Decoction is 1 -3 hours on acute inflammation in duced by carrageenan. At 1 hour,it might interfere with COX -2 pathway of arachidonic acid （AA） metabolism and reduced release of NO, TNF - α, PGE2, but at 3 hour it inhibited the release of inflammatory mediators IL - 1 β, TNF -α, PGE2 and MPO. The result re flects the time - effect relationship characteristics of Chinese Medicine Compound Guizhi Shaoyao Zhimu Decoction.