应用荧光定量PCR法联合检测NSCLC外周血有核细胞3p基因多区域LOH

目的探讨人3号染色体短臂(3p)等位基因位点杂合性缺失(LOH)多区域联合检测的意义。方法采用实时荧光定量PCR方法分别对32例非小细胞肺癌患者及8例非恶性肿瘤患者外周血有核细胞进行3p位点LOH检测,对试验结果统计学分析。结果32例非小细胞肺癌外周血有核细胞LOH联合检出率为65.625%,各目的基因检出率分别为18.75%(3p25)、31.25%(3p14)、50%(3p21.3),相比对照组检测结果差异具有显著性,联合检出率高于各位点单纯检出率,差别具有显著性。结论非小细胞肺癌外周血有核细胞3p位点LOH检测对于肺癌的无创诊断有意义,且多区域联合检测有助于提高检测的敏感性。

Research of loss of heterozygaity on chromosome 3p in peripheral blood of NSCLC by fluorescent quantitation PCR

Objective The aim of this study was to investigate the relationship between loss of heterozygosity(LOH) of the allele genes on 3p and the diagnostic value of NSCLC.Methods A total of 32 NSCLC sample and 8 pulmonary benign disease have analyzed by fluorescent quantitation-PCR.Results There were 20 of the 32 cases(65.625%) with NSCLC had LOH on 3p in the nucleated cell in peripheral blood.Among them,6 cases(18.75%) had LOH at 3p25,10cases(31.25%) had LOH at 3p14 and 16 cases(50%) had LOH at 3p21.3.Conclusion Our analysis showed that 3p LOH occur commonly in human NSCLC.The detection of the nucleated cell in peripheral blood is beneficial to the diagnosis of NSCLC.The combined LOH data of 3 separate regions of 3p LOH is beneficial to increase the detection rate.