Mitochondrial protein synthesis in interspecific somatic cell hybrids |
| |
| Authors: | S. H. Zuckerman F. P. Gillespie J. F. Solus R. Rybczynski J. M. Eisenstadt |
| |
| Affiliation: | (1) Department of Immunology, Lilly Research Laboratories, 307 E. McCarty Street, 46285 Indianapolis, Indiana;(2) Department of Human Genetics, Yale University School of Medicine, 333 Cedar Street, 06510 New Haven, Connecticut;(3) Department of Biology, University of California, 92093 San Diego, La Jolla, California;(4) Section of Molecular Neurobiology, Yale University School of Medicine, 333 Cedar Street, 06510 New Haven, Connecticut |
| |
| Abstract: | The fusion of an oligomycin (OLI) -resistant mutant of mouse LM(TK–) cells to a chloramphenicol (CAP) -resistant mutant of AK412 Chinese hamster cells resulted in a series of interspecific somatic cell hybrids. Hybrids selected in HAT medium retained only mouse mitochondrial genomes while hybrids selected in HA T plus CAP and OLI retained both hamster and mouse mitochondrial genomes in approximately equal amounts. Nuclear-coded mitochondrial proteins from both parental species were incorporated into mitochondria in all of the hybrids. However, the mitochondrially coded proteins of three individually isolated hybrid cell lines were predominantly mouse-specific, with only trace amounts of hamster protein detected. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
