内皮细胞特异性分子 1预处理对小鼠骨髓间充质干细胞生物学特性的影响

目的:探讨内皮细胞特异性分子 1（ESM 1）孵育预处理对小鼠骨髓间充质干细胞生物学特性的影响，为干细胞移植提供实验依据。方法: 培养及鉴定骨髓间充质干细胞。提取细胞数及浓度固定的初级培养液中的骨髓间充质干细胞，并分为两组:对照组（不进行预处理）及3个浓度（005 μg/ml、01 μg/ml、015 μg/ml）的ESM 1预处理组。每组设4个复孔，每孔均处理60 min。收集每孔中的条件培养液，检测预处理对骨髓间充质干细胞分泌血管内皮生长因子(VEGF)及碱性磷酸酶(ALP)的影响。结果: ESM 1孵育预处理骨髓间充质干细胞后，其存活率明显增加，而凋亡率显著下降，且骨髓间充质干细胞增殖能力随着ESM 1预处理浓度的增加而逐渐升高，凋亡率却呈相反的下降趋势。ESM 1预处理后，骨髓间充质干细胞分泌VEGF、ALP的水平明显升高，且随着ESM 1浓度的增加，骨髓间充质干细胞分泌VEGF、ALP的水平也相应增加。ESM 1孵育预处理后的骨髓间充质干细胞，细胞形态一致，生长及分化速度加快。结论: ESM 1孵育预处理骨髓间充质干细胞不仅促进骨髓间充质干细胞增殖及降低其凋亡，而且可增加VEGF、ALP的分泌。ESM 1通过活化骨髓间充质干细胞，提高细胞本身的潜能，继而提高其存活、增殖的能力, 为临床急性心肌梗死的治疗提供了广泛的应用前景。

Empirical study of bionomical effect of mice mesenchymal stem cells preconditioned by endothelial cell specific molecule 1

AIM:To investigate the endothelial cell specific molecule 1 (ESM 1) after incubation with pretreatment on mouse bone marrow mesenchymal stem cells (BM MSCs). We studied the biological characteristic influence in order to provide an experimental basis for stem cell transplantation. METHODS: For cultivation and identification of BM MSCs, extraction of cell number and concentration of fixed primary cultured BM MSCs were divided into two groups: control group (no pretreatment) and three concentrations (005 μg/ml, 01 μg/ml, 015 μg/ml) of ESM 1 pretreatment group. Each group has four complex holes. Each hole was preconditioned for 60 min. Every hole was collected in the conditioned medium, and effect of pretreatment on BM MSCs on secretion of vascular endothelial growth factor (VEGF) and alkaline phosphatase (ALP) was shown. RESULTS: After incubation of ESM 1 preconditioned BM MSCs, the survival rate increased significantly, whereas apoptosis rate decreased significantly and proliferation of BM MSCs with increasing concentrations of ESM 1 pretreatment increased, but the apoptosis rate showed the opposite decreasing trend. ESM 1 after pretreatment showed BM MSCs secreted VEGF, ALP levels increased, and with increasing the concentration of ESM 1, BM MSCs secreted VEGF. ALP levels also increased accordingly. Incubation of ESM 1 preconditioned BM MSCs, cell morphology, and growth and differentiation is accelerated. CONCLUSION: ESM 1 incubated with preconditioned BM MSCs promote the proliferation of BM MSCs and reduce apoptosis and can increase VEGF and ALP secretion. ESM 1 through the activation of BM MSCs improves the cell potential itself and then improves survival and proliferation. The prospect exists for clinical treatment of acute myocardial infarction with wide application.