| Abstract: | An interposed colon segment has been clinicallyused as a gastric substitute following anesophagogastric resection for benign or malignantesophageal and gastric cardia disease. The purpose ofthis study is to establish a rat model of colonicinterposition following distal gastrectomy and toinvestigate its serial mucosal changes. About 80% of theglandular stomach was resected, and a 3-cm segment ofthe transverse colon interposed isoperistalticallybetween the remnant stomach and duodenum. Epithelialproliferation and aberrant crypt foci in the interposedcolon segment were investigated serially. Crypt lengths in the interposed colon increasedsignificantly (P < 0.05) compared to the remnantcolon. The number of goblet cells per crypt per 1 mm inthe interposed colon also decreased significantly (P< 0.05) compared to the remnant colon. A PCNA labelingindex (LI) of the remnant colon was almost 30%. A PCNALI in the interposed colon at 4, 8, and 12 months aftersurgery was 30.8%, 31.8%, and 47.8%, respectively. The PCNA LI in the interposed colon increasedsignificantly (P < 0.05) 12 months after surgerycompared to the remnant colon at 4, 8, and 12 monthsafter surgery. Aberrant crypt foci were not detected in the interposed colon segment. In conclusion, weestablished a rat model of colonic interpositionfollowing distal gastrectomy. The interposed colonmucosa adapted well. Long-term mucosal changes of the interposed colon segment should now bestudied. |
