环7肽库筛选胃癌耐药细胞特异性结合短肽

目的获得与胃癌耐药细胞株SGC7901/VCR特异结合的抑制耐药短肽,作为提高胃癌化疗效果的先导化合物。方法以SGC7901/VCR细胞为靶细胞,胃粘膜永生化上皮细胞GES,胃癌药敏细胞SGC7901为吸附细胞对噬菌体7肽库进行消减筛选,用细胞ELISA鉴定阳性噬菌体克隆并测序,利用竞争抑制试验确定阳性克隆的结合部位是否为外源性肽段。结果经4轮筛选,从随机挑选的30个噬菌体克隆中得到14个能特异性与SGC7901/VCR结合而不与胃癌药敏细胞结合的阳性克隆,确定其氨基酸序列分别为SY1和SY2(筛选所得安基酸序列正在专利申请中),含SY1为阳性克隆。结论用噬菌体环7肽库成功筛选到能特异性结合胃癌耐药细胞株的短肽,为肿瘤治疗或药物靶向治疗奠定基础。

Ph.D. -C7CTM phage display peptide library selection on gastric cancer MDR cells to yield specific binding peptides

Objective:To obtain short peptides which specifically bind to drug resistant cells by means from 7 peptide libraries. Methods: Multidrug-resistant gastric cancer cells SGC7901/VCR were used as the target cells and GES as the absorber cells as well drug-sensitive gastric cancer cells SGC7901 for subtraction biopanning from a Ph.D.-C7C library. The positive phage clones were identified by cell enzyme-linked immunosorbent assay (ELISA), and the amino acid sequences were deduced by DNA sequencing. Results: After 4 rounds of screening, the 14 positive phage clones were identified only specific bind to the SGC-7901/VCR of human gastric cancer not to SGC-7901, DNA sequence was CTLQNRTLC and CGASNLKSC, the phages containing CTLQNRTLC were positive. Conclusion: A candidate peptide special binding to gastric resistant cancer can be selected, thus providing the potential for improving gastric tumor chemotherapy or targeted drug delivery in chemotherapy.