| 微囊藻毒素-LR的DNA损伤作用研究 |
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| 引用本文： | 吴和岩,苏瑾,施玮. 微囊藻毒素-LR的DNA损伤作用研究[J]. 卫生研究, 2005, 34(6): 690-693 |
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| 作者姓名： | 吴和岩 苏瑾 施玮 |
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| 作者单位： | 1. 复旦大学公共卫生学院,上海,200032
2. 上海市疾病预防控制中心环境卫生科 |
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| 基金项目： | 上海市科学技术委员会技术标准专项项目(02DZ505038)，卫生部卫生标准项目 |
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| 摘 要： | 目的探讨微囊藻毒素LR(MCLR)在不引起细胞毒性剂量情况下,能否引起细胞的DNA损伤,同时比较了MCLR对人肝细胞株(HL7702)和KB细胞(人口腔表皮癌细胞)的作用情况。方法利用四甲基偶氮唑盐微量酶反应比色(MTT)法测定细胞毒性,彗星试验检测细胞的DNA损伤。结果当MCLR在剂量为10～100μgL时,对这两种细胞活性无显著性影响,而HL7702细胞在30μgL时出现DNA损伤,且随着毒素剂量的增加,其DNA损伤更加明显。但在同等实验剂量下,未能见到KB细胞的DNA损伤。结论MCLR具有潜在的遗传毒性;且它导致的DNA损伤有具有胆汁酸转运系统的肝细胞系中表现出得更为显著。
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| 关 键 词： | MC-LR 慧星实验 DNA损伤 |
| 文章编号： | 1000-8020（2005）06-0690-04 |
| 收稿时间： | 2005-01-08 |
| 修稿时间： | 2005-01-08 |
Study on DNA damage induced by mierocystin-LR |
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| Wu He-yan,Su Jin,Shi Wei. Study on DNA damage induced by mierocystin-LR[J]. Journal of hygiene research, 2005, 34(6): 690-693 |
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| Authors: | Wu He-yan Su Jin Shi Wei |
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| Affiliation: | School of Public Health, Fudan University, Shanghai 200032, China |
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| Abstract: | OBJECTIVE: To investigate whether mierocystin-LR (MC-LR) at doses which were not cytotoxic can induce DNA damage and compare its effect on HL-7702 and KB cell lines. METHODS: Cytotoxin and DNA damage were detected by MTT and comet assay, respectively. RESULTS: As doses ranged from 10 to 100 micro/L, MC-LR showed no significant impact on viability of these two cell lines. However, DNA damage induced by MC-LR occurred at the dose of 30 microg/L in HL-7702 cell and significantly increased with dose. MC-LR did not induce DNA damage in KB cell. CONCLUSION: MC-LR has potential genotoxicity, DNA damage induced by MC-LR is more significant in hepatocyte with bile acid transportation system than other cell lines. |
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| Keywords: | mierocystin-LR(MC-LR) comet assay DNA damage |
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